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Literature summary extracted from

  • Seemann, M.; Tse Sum Bui, B.; Wolff, M.; Tritsch, D.; Campos, N.; Boronat, A.; Marquet, A.; Rohmer, M.
    Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein (2002), Angew. Chem. Int. Ed. Engl., 41, 4337-4339.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.3 [4Fe-4S]2+ cluster the purified protein is inactive. It is activated after reconstitution of its Fe/S cluster Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.7.3 43000
-
 x * 43000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.3 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin Escherichia coli the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.3 Escherichia coli P62620
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.3 His6-tagged enzyme. The purified protein is inactive. It is activated after reconstitution of its Fe/S cluster Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.3 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
-
 Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?
1.17.7.3 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.7.3 ? x * 43000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.17.7.3 GcpE ambiguous Escherichia coli

General Information

EC Number General Information Comment Organism
1.17.7.3 metabolism the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis Escherichia coli