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Literature summary extracted from
- Examination of PHB depolymerases in Ralstonia eutropha: further elucidation of the roles of enzymes in PHB homeostasis (2012), AMB Express, 2, 26.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.75 | gene phaZ1 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ2 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ3 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ4 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ5 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ6 | Cupriavidus necator |
| 3.1.1.75 | gene phaZ7 | Cupriavidus necator |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | additional information | construction of a single knockout mutant or double and quadruple knockout mutants | Cupriavidus necator |
| 3.1.1.75 | additional information | construction of a single knockout mutant or double and quadruple knockout mutants, complementation analysis of Ralstonia eutropha strain Re2005 (DELTAphaZ1DELTAphaZ2DELTAphaZ3DELTAphaZ5) cells | Cupriavidus necator |
| 3.1.1.75 | additional information | construction of a single knockout mutant or double and quadruple knockout mutants, for deletion of the phaZ3 gene, Ralstonia eutropha strains H16 and Re1111 are used as parental strains, complementation analysis of Ralstonia eutropha strain Re2005 (DELTAphaZ1DELTAphaZ2DELTAphaZ3DELTAphaZ5) cells | Cupriavidus necator |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.75 | intracellular | - |
Cupriavidus necator | 5622 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | Cupriavidus necator | - |
? | - |
? |  |
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.75 | Cupriavidus necator | Q0JYJ1 | gene phaZ7; gene phaZ7 | - |
| 3.1.1.75 | Cupriavidus necator | Q0JZG9 | gene phaZ6; gene phaZ6 | - |
| 3.1.1.75 | Cupriavidus necator | Q0K2G9 | gene phaZ5; gene phaZ5 | - |
| 3.1.1.75 | Cupriavidus necator | Q0K4D5 | gene phaZ3; gene phaZ3 | - |
| 3.1.1.75 | Cupriavidus necator | Q0K7T2 | gene phaZ2; gene phaZ2 | - |
| 3.1.1.75 | Cupriavidus necator | Q0KCI0 | gene phaZ1; gene phaZ1 | - |
| 3.1.1.75 | Cupriavidus necator | Q7WXF6 | gene phaZ4; gene phaZ4 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0JYJ1 | gene phaZ7; gene phaZ7 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0JZG9 | gene phaZ6; gene phaZ6 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K2G9 | gene phaZ5; gene phaZ5 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K4D5 | gene phaZ3; gene phaZ3 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K7T2 | gene phaZ2; gene phaZ2 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KCI0 | gene phaZ1; gene phaZ1 | - |
| 3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q7WXF6 | gene phaZ4; gene phaZ4 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | - |
Cupriavidus necator | ? | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-4-hydroxybutyrate) + H2O | - |
Cupriavidus necator | ? | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-4-hydroxybutyrate) + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | PHA depolymerase | - |
Cupriavidus necator |
| 3.1.1.75 | PhaZ | - |
Cupriavidus necator |
| 3.1.1.75 | PHB depolymerase | - |
Cupriavidus necator |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | malfunction | a phaZ quadruple knockout strain, lacking genes phaZ1, phaZ2, phaZ3, and phaZ5, appears to have a low granule volume per cell volume and a low granule count per cell | Cupriavidus necator |
| 3.1.1.75 | malfunction | a phaZ quadruple knockout strain, lacking genes phaZ1, phaZ2, phaZ3, and phaZ5, appears to have a low granule volume per cell volume and a low granule count per cell. Cells bearing a phaZ3 knockout are found to have a higher granule count than the wild-type, whereas granule volume per cell volume is similar | Cupriavidus necator |
| 3.1.1.75 | malfunction | cells bearing a phaZ2 knockout mutation alone or in conjunction with a phaZ1 mutation are found to have a high granule volume per cell volume and a higher granule count compared to wild-type. A phaZ quadruple knockout strain, lacking genes phaZ1, phaZ2, phaZ3, and phaZ5, appears to have a low granule volume per cell volume and a low granule count per cell. Cells bearing a phaZ3 knockout are found to have a higher granule count than the wild-type, whereas granule volume per cell volume is similar | Cupriavidus necator |
| 3.1.1.75 | physiological function | the enzyme has an impact on granule architecture. Overexpression of specific phaZ genes influences polyhydroxybutyrate homeostasis | Cupriavidus necator |
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