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Literature summary extracted from

  • Zhang, L.; Zhao, P.; Chen, C.C.; Huang, C.H.; Ko, T.P.; Zheng, Y.; Guo, R.T.
    Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum (2014), Acta Crystallogr. Sect. F, 70, 946-948.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression of the His-tagged isolated catalytic domain of the enzyme, residues 31-251, in Escherichia coli strain BL21trxB(DE3) with an extra linker AGAGA at its N-terminus Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.73 purified recombinant catalytic domain, sitting drop vapour diffusion method, mixing of 0.002 ml of 30 mg/ml protein in 25 mM Tris pH 7.5, 150 mM NaCl, and 0.002 ml reservori solution containing 0.04 M citric acid, 0.06 M Bis-Tris propane, pH 6.4, 18% w/v PEG 3350, 25°C, 7 days, optimized method, X-ray diffraction structure determination and analysis at 1.95 A resolution, molecular replacement method Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 28000
-
 x * 28000, about, recombinant catalytic domain, SDS-PAGE Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.73 additional information Acetivibrio thermocellus the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates ?
-
 ?
3.2.1.73 additional information Acetivibrio thermocellus DSM 1237 the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Acetivibrio thermocellus A3DBX3
-
-
3.2.1.73 Acetivibrio thermocellus DSM 1237 A3DBX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 recombinant His-tagged isolated catalytic domain of the enzyme from Escherichia coli strain BL21trxB(DE3) by nickel affinity chromatography and dialysis, tag cleavage by factor Xa digestion, and removal of the tag by another step of nickel affinity chromatography Acetivibrio thermocellus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.73 beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose + 4 H2O = 5 beta-D-glucopyranose beta-1,3-1,4-glucanase catalyzes strict endo-hydrolysis of the beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in the mixed-linked beta-glucans via a double-displacement mechanism Acetivibrio thermocellus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
3.2.1.73 additional information the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates Acetivibrio thermocellus ?
-
 ?
3.2.1.73 additional information the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates Acetivibrio thermocellus DSM 1237 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 28000, about, recombinant catalytic domain, SDS-PAGE Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3-1,4-glucanase
-
 Acetivibrio thermocellus
3.2.1.73 CtGlu16A
-
 Acetivibrio thermocellus
3.2.1.73 Lichenase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 80
-
-
 Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 8 9
-
 Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5 12 50% of maximal activity within this range Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
3.2.1.73 evolution beta-1,3-1,4-glucanases from various microorganisms and plants belong to glycoside hydrolase families 16 and 17, respectively Acetivibrio thermocellus