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Literature summary extracted from

  • Singh, N.; Halliday, A.C.; Knight, M.; Lack, N.A.; Lowe, E.; Churchill, G.C.
    Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens (2012), Acta Crystallogr. Sect. F, 68, 1149-1152.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in Escherichia coli BLR (DE3) pLysS cells Homo sapiens
3.1.3.25 expressed in Escherichia coli BLR (DE3) pLysS cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.25 sitting drop vapor diffusion method, using 40% (w/v) PEG 3350, 0.2 M magnesium formate, at 22°C Homo sapiens
3.1.3.25 sitting drop vapor diffusion method, using 40% (w/v) PEG 3350, 0.2 M magnesium formate, at 22°C Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
 Homo sapiens
3.1.3.25 Li+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol phosphate + H2O Mus musculus
-
 myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Homo sapiens P29218
-
-
3.1.3.25 Mus musculus O55023
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 Q Sepharose column chromatography, Mono Q column chromatography, and Sephadex 75 gel filtration Homo sapiens
3.1.3.25 Q Sepharose column chromatography, Mono Q column chromatography, and Sephadex 75 gel filtration Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 myo-inositol phosphate + H2O
-
 Mus musculus myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase 1
-
 Homo sapiens
3.1.3.25 IMPase 1
-
 Mus musculus
3.1.3.25 inositol monophosphatase
-
 Homo sapiens
3.1.3.25 inositol monophosphatase
-
 Mus musculus