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Literature summary extracted from

  • Zhu, A.Y.; Zhou, Y.; Khan, S.; Deitsch, K.W.; Hao, Q.; Lin, H.
    Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine (2012), ACS Chem. Biol., 7, 155-159.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.B41 medicine PfSir2A inhibitors may have therapeutic value in malaria treatment Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B41
-
 Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.B41 hanging drop vapor diffusion method, cocrystal of enzyme with [histone H3 peptide]-N6-myristoyl-L-lysine Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B41 0.0012
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.008
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.039
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B41 Plasmodium falciparum Q8IE47
-
-
2.3.1.B41 Plasmodium falciparum isolate 3D7 Q8IE47
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B41
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B41 Sir2A
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B41 37
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.B41 0.001
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.001
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.001
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.01
-
 [histone H3 peptide]-N6-myristoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B41 8
-
 assay at Plasmodium falciparum

General Information

EC Number General Information Comment Organism
2.3.1.B41 physiological function the enzyme regulates the expression of surface antigens to evade the detection by host immune surveillance. The physiological function of PfSir2A in antigen variation may be achieved by removing medium and long chain fatty acyl groups from protein lysine residues Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.B41 0.026
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.16
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 0.92
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
2.3.1.B41 10
-
 [histone H3 peptide]-N6-myristoyl-L-lysine kcat/KM is above 10/s*mM, pH 8.0, 37°C Plasmodium falciparum