EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.1 | expressed as a His-tagged fusion protein | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.3.1 | refined to 2.5 A. The structure of MtTS has a homodimeric organization in which the two subunits are related by a non-crystallographic 2fold axis. Each subunit is composed of three domains | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.1 | additional information | a deletion of the MtTS gene generated a strain of Mycobacterium tuberculosis that requires threonine for growth | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.1 | 1.2 | - |
O-phospho-L-homoserine | - |
Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.1 | Mycobacterium tuberculosis | P9WG59 | - |
- |
4.2.3.1 | Mycobacterium tuberculosis H37Rv | P9WG59 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.1 | using Ni-NTA chromatography | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.3.1 | O-phospho-L-homoserine + H2O = L-threonine + phosphate | analyzed crystal structure gives new insights into the catalytic mechanism of threonine synthase in general, specifically by suggesting the direct involvement of the phosphate moiety of the cofactor, rather than the inorganic phosphate product, in transferring a proton from C4 to Cgamma in the formation of the alphabeta-unsaturated aldimine | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 1.05 | - |
- |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.1 | O-phospho-L-homoserine + H2O | - |
Mycobacterium tuberculosis | L-threonine + phosphate | - |
? | |
4.2.3.1 | O-phospho-L-homoserine + H2O | - |
Mycobacterium tuberculosis H37Rv | L-threonine + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.3.1 | homodimer | crystal structure | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.1 | MtTS | - |
Mycobacterium tuberculosis |
4.2.3.1 | threonine synthase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 25 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 60 | - |
MtTS loses less than 10% of the initial activity during a 10 min incubation | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.1 | 7.3 | - |
O-phospho-L-homoserine | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 8.4 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 7 | - |
MtTS is inactivated at pH 7 or below | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.1 | pyridoxal 5'-phosphate | MtTS is not influenced by S-adenosylmethionine | Mycobacterium tuberculosis |