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Literature summary extracted from

  • Chao, O.Y.; Gao, S.; Cai, F.; Chung, T.W.; Wang, S.H.; Chen, F.
    Cloning, expression, and purification of a Cu/Zn superoxide dismutase from Jatropha curcas (2013), Z. Naturforsch. C, 68, 60-69.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 expression of His6-and thioredoxin-tagged enzyme in Escherichia coli Jatropha curcas

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 2-mercaptoethanol 8 mM, slight inhibition Jatropha curcas
1.15.1.1 EDTA at 16 mM Jatropha curcas
1.15.1.1 guanidinium hydrochloride 6 M, slight inhibition Jatropha curcas
1.15.1.1 H2O2 at 16 mM Jatropha curcas
1.15.1.1 imidazole 1 mM, slight inhibition Jatropha curcas
1.15.1.1 Urea 8 M, slight inhibition Jatropha curcas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu/Zn-SOD, the enzyme contains 1.54 mg/l copper atoms, and 0.239 mol Cu2+ per mol of enzyme Jatropha curcas
1.15.1.1 Zn2+ a Cu/Zn-SOD, the enzyme contains 1.71 mg/l zinc atoms, 0.258 mol Zn2+ per mol of enzyme Jatropha curcas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 16385
-
 1 * 16385, sequence calculation, 1 * 16400, about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry and SDS-PAGE Jatropha curcas
1.15.1.1 16400
-
 about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry Jatropha curcas
1.15.1.1 16500
-
 recombinant His6-and thioredoxin-tagged enzyme, gel filtration Jatropha curcas

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Jatropha curcas H8YHP3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant His6-and thioredoxin-tagged enzyme from Escherichia coli, 12.2fold by two steps of nickel affinity chromatography and desalting gel filtration Jatropha curcas

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 151.9
-
 purified recombinant enzyme, pH and temperature not specified in the publication Jatropha curcas

Subunits

EC Number Subunits Comment Organism
1.15.1.1 monomer 1 * 16385, sequence calculation, 1 * 16400, about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry and SDS-PAGE Jatropha curcas

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Cu/Zn superoxide dismutase
-
 Jatropha curcas
1.15.1.1 Cu/Zn-SOD
-
 Jatropha curcas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 30 60 about 80% activity remaining after 60 min Jatropha curcas
1.15.1.1 70
-
 50% activity remaining after 30 min, inactivation after 60 min Jatropha curcas

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Jatropha curcas isoelectric focusing reveals four isoelectric variants in the recombinant JcCu/Zn-SOD, with pI values of 7.04, 7.33, 8.62, and 8.77
-
 additional information
1.15.1.1 Jatropha curcas isoelectric focusing, recombinant JcCu/Zn-SOD
-
 7.04
1.15.1.1 Jatropha curcas isoelectric focusing, recombinant JcCu/Zn-SOD
-
 7.33
1.15.1.1 Jatropha curcas isoelectric focusing, recombinant JcCu/Zn-SOD
-
 8.62
1.15.1.1 Jatropha curcas isoelectric focusing, recombinant JcCu/Zn-SOD
-
 8.77