EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.9 | expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermococcus kodakarensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.9 | EDTA | the enzyme activity is completely diminished when the enzyme is heated at 70°C in the presence of 10 mM EDTA. Complete restoration of the enzyme activity is observed when the enzyme is incubated at room temperature in the presence of Zn2+ | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.9 | 2.2 | - |
D-fructose 6-phosphate | at pH 6.0 and 37°C | Thermococcus kodakarensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.9 | Fe2+ | Fe2+ also enhances the enzyme activity but to a lesser extent than Zn2+ | Thermococcus kodakarensis | |
5.3.1.9 | Mn2+ | Mn2+ also enhances the enzyme activity but to a lesser extent than Zn2+ | Thermococcus kodakarensis | |
5.3.1.9 | Zn2+ | addition of Zn2+ ions remarkably enhances the enzyme activity (more than 3fold at 0.2 mM) | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.9 | 24000 | - |
x * 24000, SDS-PAGE | Thermococcus kodakarensis |
5.3.1.9 | 24675 | - |
x * 24675, calculated from amino acid sequence | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.9 | D-fructose 6-phosphate | Thermococcus kodakarensis | - |
D-glucose 6-phosphate | - |
r | |
5.3.1.9 | D-glucose 6-phosphate | Thermococcus kodakarensis | - |
D-fructose 6-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.9 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.9 | heat precipitation and Resource Q column chromatography | Thermococcus kodakarensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.9 | 43 | - |
with D-fructose 6-phosphate as substrate, at 90°C and pH 6.0 | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.9 | D-fructose 6-phosphate | - |
Thermococcus kodakarensis | D-glucose 6-phosphate | - |
r | |
5.3.1.9 | D-glucose 6-phosphate | - |
Thermococcus kodakarensis | D-fructose 6-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.9 | ? | x * 24000, SDS-PAGE | Thermococcus kodakarensis |
5.3.1.9 | ? | x * 24675, calculated from amino acid sequence | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.9 | Phosphoglucose isomerase | - |
Thermococcus kodakarensis |
5.3.1.9 | TK1111 | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.9 | 90 | - |
- |
Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.9 | 90 | - |
the enzyme exhibits a half-life of 40 min at 90°C | Thermococcus kodakarensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.9 | 5 | - |
D-fructose 6-phosphate | at pH 6.0 and 37°C | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.9 | 6 | - |
- |
Thermococcus kodakarensis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.3.1.9 | Thermococcus kodakarensis | calculated from amino acid sequence | - |
5.1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.9 | 2.27 | - |
D-fructose 6-phosphate | at pH 6.0 and 37°C | Thermococcus kodakarensis |