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Literature summary extracted from

  • Chaudhuri, B.N.; Lange, S.C.; Myers, R.S.; Chittur, S.V.; Davisson, V.J.; Smith, J.L.
    Crystal structure of imidazole glycerol phosphate synthase: a tunnel through a (beta/alpha)8 barrel joins two active sites (2001), Structure, 9, 987-997.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.B2 crystallization at 20°C by hanging-drop vapor diffusion from a 1:1 mixture of protein solution and reservoir solution [22%–28% polyethylene glycol MME 5000, 0.1 M MES (pH 6.5–7.0), and 0.2 M (NH4)2SO4]. Crystals grow in 7–10 days to an average size of 0.12 * 0.12 * 0.08 mM. The 2.1 A crystal structure of imidazole glycerol phosphate synthase reveals extensive interaction of the glutaminase and cyclase catalytic domains. At the domain interface, the glutaminase active site points into the bottomof the (beta/alpha)8 barrel of the cyclase domain. An ammonia tunnel through the (beta/alpha)8 barrel connects the glutaminase docking site at the bottom to the cyclase active site at the top. A conserved gate of four charged residues controls access to the tunnel Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.B2 acivicin
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.B2 Saccharomyces cerevisiae P33734
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.B2
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
4.3.1.B2 NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.B2 IGPS
-
 Saccharomyces cerevisiae