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Literature summary extracted from

  • Broussard, T.C.; Kobe, M.J.; Pakhomova, S.; Neau, D.B.; Price, A.E.; Champion, T.S.; Waldrop, G.L.
    The three-dimensional structure of the biotin carboxylase-biotin carboxyl carrier protein complex of E. coli acetyl-CoA carboxylase (2013), Structure, 21, 650-657.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.14 in complex with biotinylated biotin carboxyl carrier protein, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 6.5), and 25% (w/v) PEG 3350 Escherichia coli
6.4.1.2 crystal structure of Escherichia coli biotinylated biotin carboxyl carrier protein (BCCP) complexed with biotin carboxylase (BC) to a resolution of 2.49 A. The protein-protein complex shows a unique quaternary structure and two distinct interfaces for each biotinylated biotin carboxyl carrier protein monomer Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 50000
-
 2 * 50000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- Escherichia coli
-
 ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli P24182
-
-
6.4.1.2 Escherichia coli P0ABD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14 nickel affinity chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 homodimer 2 * 50000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 acetyl-CoA carboxylase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP
-
 Escherichia coli