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Literature summary extracted from

  • Koh, C.Y.; Kim, J.E.; Shibata, S.; Ranade, R.M.; Yu, M.; Liu, J.; Gillespie, J.R.; Buckner, F.S.; Verlinde, C.L.; Fan, E.; Hol, W.G.
    Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection (2012), Structure, 20, 1681-1691.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.10 in complex with L-methionine and inhibitors, sitting drop vapor diffusion method, using 2.0-2.3 M (NH4)2SO4, 0.2 M NaCl, and 0.1 M sodium cacodylate (pH 6.0-6.6) Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
-
 Trypanosoma brucei
6.1.1.10 2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
-
 Trypanosoma brucei
6.1.1.10 2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one
-
 Trypanosoma brucei
6.1.1.10 2-aminoquinolin-8-ol
-
 Trypanosoma brucei
6.1.1.10 4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one
-
 Trypanosoma brucei
6.1.1.10 N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Trypanosoma brucei
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Trypanosoma brucei Q38C91
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 Ni-NTA affinity column chromatography and Superdex 75 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Trypanosoma brucei AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 methionyl-tRNA synthetase
-
 Trypanosoma brucei
6.1.1.10 MetRS
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.10 0.000008
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one
6.1.1.10 0.000013
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
6.1.1.10 0.000043
-
 pH and temperature not specified in the publication Trypanosoma brucei 4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one
6.1.1.10 0.000046
-
 pH and temperature not specified in the publication Trypanosoma brucei N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine
6.1.1.10 0.000065
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one