EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.67 | crystal structure resolved to 1.8 A resolution | Escherichia coli |
3.6.1.67 | the structure of the enzyme is determined by X-ray diffraction, 1.8 A resolution. The best crystals grow by microseeding in sitting-drop experiments with a 1 ml reservoir solution of 1.31.5 M ammonium sulfate, 1% propanol, 35 mM DTT, 4 mM sodium diphosphate, 100 mM Na HEPES (pH 6.8). Crystals belong to monoclinic space group C2 with cell dimensions a = 124.1 A, b = 43.2 A, c = 108.0 A, and b = 115.0° | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.67 | 0.27 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.5, 37°C | Escherichia coli | |
3.6.1.67 | 0.79 | - |
dATP | pH 8.5, 37°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.67 | 7,8-dihydroneopterin 3'-triphosphate + H2O | Escherichia coli | committed step of folate synthesis in bacteria | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
3.6.1.67 | 7,8-dihydroneopterin 3'-triphosphate + H2O | Escherichia coli | key enzyme in the folate pathway | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.67 | Escherichia coli | P0AFC0 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.67 | 3 | 8 | pH 8.5, 37°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.67 | 7,8-dihydroneopterin 3'-triphosphate + H2O | - |
Escherichia coli | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
3.6.1.67 | 7,8-dihydroneopterin 3'-triphosphate + H2O | committed step of folate synthesis in bacteria | Escherichia coli | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
3.6.1.67 | 7,8-dihydroneopterin 3'-triphosphate + H2O | key enzyme in the folate pathway | Escherichia coli | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
3.6.1.67 | dATP + H2O | - |
Escherichia coli | dAMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.67 | DHNTPase | - |
Escherichia coli |
3.6.1.67 | dihydroneopterin triphosphate pyrophosphatase | - |
Escherichia coli |
3.6.1.67 | nudB | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.67 | 37 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.67 | 4.59 | - |
dATP | pH 8.5, 37°C | Escherichia coli | |
3.6.1.67 | 11.6 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.5, 37°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.67 | 8.5 | - |
assay at | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.1.67 | malfunction | knockout of this gene in Escherichia coli leads to a marked reduction in folate synthesis that is completely restored by a plasmid carrying the gene | Escherichia coli |
3.6.1.67 | metabolism | metabolism | Escherichia coli |
3.6.1.67 | physiological function | committed step of folate synthesis in bacteria | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.67 | 5.8 | - |
dATP | pH 8.5, 37°C | Escherichia coli | |
3.6.1.67 | 43 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.5, 37°C | Escherichia coli |