EC Number | Cloned (Comment) | Organism |
---|---|---|
6.5.1.3 | expressed in Escherichia coli as a His-tagged fusion protein | Tequatrovirus T4 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.3 | Tequatrovirus T4 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.3 | using Ni-NTA chromatography | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m | the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs | Tequatrovirus T4 | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.5.1.3 | Rnl2 | - |
Tequatrovirus T4 |
6.5.1.3 | T4 RNA ligase 2 | - |
Tequatrovirus T4 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.5.1.3 | additional information | - |
additional information | the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs | Tequatrovirus T4 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.3 | 6.8 | - |
assay at | Tequatrovirus T4 |