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Literature summary extracted from

  • Hayes, J.; Skamnaki, V.; Archontis, G.; Lamprakis, C.; Sarrou, J.; Bischler, N.; Skaltsounis, A.; Zographos, S.; Oikonomakos, N.
    Kinetics, in silico docking, molecular dynamics, and MM-GBSA binding studies on prototype indirubins, KT5720, and staurosporine as phosphorylase kinase ATP-binding site inhibitors: The role of water molecules examined (2011), Proteins, 79, 703-719.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.19 expressed as a N-terminal GST fusion, expressed in B834 (DE3)pLyS cells at 18°C Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 indirubin phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
2.7.11.19 indirubin-3'-oxime phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
2.7.11.19 KT5720 phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
2.7.11.19 staurosporine phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 2 ATP + glycogen phosphorylase b
-
 Oryctolagus cuniculus 2 ADP + glycogen phosphorylase a
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 8.2
-
 assay at Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.19 0.00000037
-
 KT5720 30°C, pH 8.2 Oryctolagus cuniculus
2.7.11.19 0.0000184
-
 staurosporine 30°C, pH 8.2 Oryctolagus cuniculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.19 0.00000037
-
 30°C, pH 8.2 Oryctolagus cuniculus staurosporine
2.7.11.19 0.0000184
-
 30°C, pH 8.2 Oryctolagus cuniculus KT5720
2.7.11.19 0.000144
-
 30°C, pH 8.2 Oryctolagus cuniculus indirubin-3'-oxime
2.7.11.19 0.05
-
 30°C, pH 8.2 Oryctolagus cuniculus indirubin