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Literature summary extracted from

  • Yu, T.Y.; Mok, K.C.; Kennedy, K.J.; Valton, J.; Anderson, K.S.; Walker, G.C.; Taga, M.E.
    Active site residues critical for flavin binding and 5,6-dimethylbenzimidazole biosynthesis in the flavin destructase enzyme BluB (2012), Protein Sci., 21, 839-849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.79 gene bluB, genotyping Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.79 A156V site-directed mutagenesis, inactive mutant Sinorhizobium meliloti
1.13.11.79 D32N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Sinorhizobium meliloti
1.13.11.79 D32N/S167G site-directed mutagenesis, the mutant shows no activity Sinorhizobium meliloti
1.13.11.79 E78K site-directed mutagenesis, the mutant is not soluble Sinorhizobium meliloti
1.13.11.79 G110S site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 G133S site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 G193D site-directed mutagenesis, inactive mutant Sinorhizobium meliloti
1.13.11.79 G61D site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 M140I site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Sinorhizobium meliloti
1.13.11.79 M94I site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Sinorhizobium meliloti
1.13.11.79 P202L site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 P65L site-directed mutagenesis, inactive mutant Sinorhizobium meliloti
1.13.11.79 R30C site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 R30H site-directed mutagenesis, the mutant shows no 5,6-dimethylbenzimidazole forming activity Sinorhizobium meliloti
1.13.11.79 S167G site-directed mutagenesis, the mutant shows higly reduced activity compared to the wild-type enzyme Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.79 additional information
-
additional information FMN/FMNH2 dissociation constants and free energy of binding of wild-type and mutant enzymes, overview Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.79 FMNH2 + NADH + H+ + O2 Sinorhizobium meliloti
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ? 5,6-dimethylbenzimidazole is the lower axial ligand of vitamin B12, i.e. cobalamin ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.79 Sinorhizobium meliloti Q92PC8 gene bluB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.79 FMNH2 + NADH + H+ + O2
-
Sinorhizobium meliloti 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ? 5,6-dimethylbenzimidazole is the lower axial ligand of vitamin B12, i.e. cobalamin ?
1.13.11.79 FMNH2 + NADH + H+ + O2 BluB fragments a flavin isoalloxazine ring Sinorhizobium meliloti 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.79 BluB
-
Sinorhizobium meliloti
1.13.11.79 flavin destructase
-
Sinorhizobium meliloti
1.13.11.79 flavin destructase enzyme
-
Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.79 NADH
-
Sinorhizobium meliloti

General Information

EC Number General Information Comment Organism
1.13.11.79 additional information identification of catalytic residues involved in the reaction, mutational analysis, overview. The enzyme shows interactions with the phosphate group and ribityl tail of FMN Sinorhizobium meliloti