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Literature summary extracted from

  • Nomura, Y.; Onda, Y.; Ohno, S.; Taniguchi, H.; Ando, K.; Oka, N.; Nishikawa, K.; Yokogawa, T.
    Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans (2013), Protein Expr. Purif., 88, 13-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.29 overexpression in Escherichia coli Methanosarcina acetivorans
2.4.2.48 expressed in Escherichia coli ER2566 cells Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.29 additional information
-
 additional information kinetic parameters of recombinant and native enzyme Methanosarcina acetivorans
2.4.2.48 0.0012
-
 guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
2.4.2.48 0.0021
-
 guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.29 Mg2+ optimal concentration : 10 mM Methanosarcina acetivorans
2.4.2.48 Mg2+ maximum activity at 10 mM Mg2+ Methanosarcina acetivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.29 19000
-
 2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT Methanosarcina acetivorans
2.4.2.29 54000
-
 2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT Methanosarcina acetivorans
2.4.2.29 150000
-
 gel filtration Methanosarcina acetivorans
2.4.2.48 19000
-
-
 Methanosarcina acetivorans
2.4.2.48 54000
-
-
 Methanosarcina acetivorans
2.4.2.48 150000
-
 gel filtration Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine Methanosarcina acetivorans
-
 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine Methanosarcina acetivorans DSM 2834
-
 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Methanosarcina acetivorans Q8THU2 and Q8TUE6 Q8THU2: Q8TUE6:
-
2.4.2.29 Methanosarcina acetivorans DSM 2834 Q8THU2 and Q8TUE6 Q8THU2: Q8TUE6:
-
2.4.2.48 Methanosarcina acetivorans Q8THU2
-
-
2.4.2.48 Methanosarcina acetivorans DSM 2834 Q8THU2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.29
-
 Methanosarcina acetivorans
2.4.2.48 ammonium sulfate precipitation, phenyl Sepharose column chromatography, HiTrap Q column chromatography, HiTrap heparin column chromatography, Mono Q column chromatography, and Sephacryl S-200 gel filtration Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.48 27
-
 native enzyme, after 2900fold purification, at pH 7.5 and 37°C Methanosarcina acetivorans
2.4.2.48 31
-
 recombinant enzyme, after 14fold purification, at pH 7.5 and 37°C Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine
-
 Methanosarcina acetivorans 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine
-
 Methanosarcina acetivorans DSM 2834 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.29 heterotetramer 2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT Methanosarcina acetivorans
2.4.2.48 heterotetramer 2 * 54000 + 2 * 19000 Methanosarcina acetivorans

Synonyms

EC Number Synonyms Comment Organism
2.4.2.29 ArcTGT
-
 Methanosarcina acetivorans
2.4.2.29 tRNA-guanine transglycosylase
-
 Methanosarcina acetivorans
2.4.2.48 archaeosine tRNAguanine transglycosylase
-
 Methanosarcina acetivorans
2.4.2.48 ArcTGT
-
 Methanosarcina acetivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.29 37
-
 assay at Methanosarcina acetivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.48 0.0311
-
 guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
2.4.2.48 0.0667
-
 guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.29 7.5
-
-
 Methanosarcina acetivorans
2.4.2.48 7.5
-
-
 Methanosarcina acetivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.29 7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 50% oif maximal activity Methanosarcina acetivorans

General Information

EC Number General Information Comment Organism
2.4.2.29 physiological function in the first step of archaeosine biosynthesis, archaeosine tRNAguanine transglycosylase catalyzes the base exchange reaction from guanine to 7-cyano-7-deazaguanine Methanosarcina acetivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.48 27
-
 guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
2.4.2.48 31
-
 guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans