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Literature summary extracted from

  • Wang, Y.; Xie, J.J.; Han, Z.; Liu, J.H.; Liu, X.P.
    Expression, purification and biochemical characterization of Methanocaldococcus jannaschii DNA ligase (2013), Protein Expr. Purif., 87, 79-86.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 MgCl2 50 mM Methanocaldococcus jannaschii
6.5.1.1 additional information the effects of mismatches on joining short oligonucleotides by Methanocaldococcus jannaschii DNA ligase are fully characterized. The mismatches at the first position 5' to the nick inhibits ligation more than those at the first position 3' to the nick. The mismatches at other positions 5' to the nick (3rd to 7th sites) exhibits less inhibition on ligation. However, the introduction of a C/C mismatch at the third position 5' to the nick completely inhibits the ligation of the terminal-mismatched nick of an oligonucleotide duplex by the DNA ligase Methanocaldococcus jannaschii
6.5.1.1 NaCl
-
 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ exhibits some activation on ligation Methanocaldococcus jannaschii
6.5.1.1 Mg2+ dependent on metal ions of Mg2+ and Mn2+. Ligation activity with a 2 to 25 mM concentration, optimal Mg2+ concentration is 10 mM Methanocaldococcus jannaschii
6.5.1.1 Mn2+ dependent on metal ions of Mg2+ and Mn2+ Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Methanocaldococcus jannaschii Q57635
-
-
6.5.1.1 Methanocaldococcus jannaschii DSM 2661 Q57635
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP is the best nucleotide cofactor for Methanocaldococcus jannaschii DNA ligase. dATP also exhibits some activation on ligation. All other nucleotide cofactors, including NAD+, NADH, UTP, CTP, GTP, dTTP, dCTP, and dGTP, play no role in ligation by the enzyme Methanocaldococcus jannaschii AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP is the best nucleotide cofactor for Methanocaldococcus jannaschii DNA ligase. dATP also exhibits some activation on ligation. All other nucleotide cofactors, including NAD+, NADH, UTP, CTP, GTP, dTTP, dCTP, and dGTP, play no role in ligation by the enzyme Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 MJ0171 locus name Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 50
-
 assay at Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.1 37 60 ligation activity at temperatures ranging from 37°C to 60°C Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.1 90
-
 20 min, about 20% loss of activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.5
-
 assay at Methanocaldococcus jannaschii
6.5.1.1 8.5
-
-
 Methanocaldococcus jannaschii