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Literature summary extracted from
- Expression and purification of recombinant human tyrosine hydroxylase as a fusion protein in Escherichia coli (2012), Protein Expr. Purif., 84, 219-223.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.16.2 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.2 | 3,4-dihydroxyphenylacetaldehyde | potent inhibitor | Homo sapiens |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | 56000 | - |
x * 56000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | Homo sapiens | - |
3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.2 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.2 | amylose resin column chromatography and HiPrep Q column chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | 0.0054 | - |
crude enzyme, at pH 6.8 and 37°C | Homo sapiens |
| 1.14.16.2 | 0.0163 | - |
purified enzyme, at pH 6.8 and 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.2 | ? | x * 56000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.16.2 | tyrosine hydroxylase | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.2 | tetrahydrobiopterin | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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