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Literature summary extracted from

  • Sun, F.; Zhang, X.Z.; Myung, S.; Zhang, Y.H.
    Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: optimized heat treatment purification and basic characterization (2012), Protein Expr. Purif., 82, 302-307.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

General Stability

EC Number General Stability Organism
5.3.1.6 maximum activities in sodium dihydrogen phosphate-ciitric acid buffer at pH 6.5-8.0 and in 50 mM Tris-HCl buffer pH 7.0-9.0. This enzyme retains about 65% activity from pH 8.8-11.0 in glycine-NaOH buffer Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.6 1 2.1 D-ribose 5-phosphate at 80°C and pH 7.0 Thermotoga maritima
5.3.1.6 7.6
-
 D-ribose 5-phosphate at 60°C and pH 7.0 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 15867
-
 x * 15867, calculated from amino acid sequence Thermotoga maritima
5.3.1.6 16000
-
 x * 16000, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Thermotoga maritima
-
 D-ribulose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 heat precipitation Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.6 187
-
 cell lysate, at 50°C and pH 7.0 Thermotoga maritima
5.3.1.6 290
-
 after purification, at 50°C and pH 7.0 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate
-
 Thermotoga maritima D-ribulose 5-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.6 ? x * 16000, SDS-PAGE Thermotoga maritima
5.3.1.6 ? x * 15867, calculated from amino acid sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose-5-phosphate isomerase type B
-
 Thermotoga maritima
5.3.1.6 RpiB
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.6 70
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.6 40 99 enzyme activity increases significantly when the temperature increases until 80°C. The optimum temperature is 80°C. The activity at 30°C is 25% of that at 80°C. The enzyme activity decreases after 80°C. It is still highly active when the temperature reaches 99°C, 90% activity of that of 80°C. This enzyme shows relatively high activity (above 50% of its maximum activity) over a large temperature range of 40-99°C Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.6 60 80 the purified enzyme at 0.1 mg/ml is very stable at 60 and 70°C but is deactivated at 80°C. The enzyme shows half lifetimes of 70.8, 53.1, 6.4, and 1.2 h at 60, 70, 80, and 90°C, respectively Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.6 540
-
 D-ribose 5-phosphate at 60°C and pH 7.0 Thermotoga maritima
5.3.1.6 1192
-
 D-ribose 5-phosphate at 80°C and pH 7.0 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.6 6.5 8
-
 Thermotoga maritima