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Literature summary extracted from

  • Li, Y.; Sousa, R.
    Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation (2012), Protein Expr. Purif., 82, 162-167.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Escherichia coli
-
-
-
6.3.4.15 Escherichia coli AVB101
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 HisTrap column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + Avi-tagged thioredoxin biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) Escherichia coli ?
-
 ?
6.3.4.15 ATP + biotin + Avi-tagged thioredoxin biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) Escherichia coli AVB101 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 ? x * about 35000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin ligase
-
 Escherichia coli
6.3.4.15 BirA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Escherichia coli