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Literature summary extracted from

  • Syed, K.; Porollo, A.; Miller, D.; Yadav, J.
    Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons (2013), Protein Eng., 26, 553-557.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 expressed in Pichia pastoris strain KM71H Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.1 W129A/L324G the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (32%) toward phenanthrene Phanerodontia chrysosporium
1.14.14.1 W129F/L324F the mutant shows complete loss of activity toward both phenanthrene and pyrene Phanerodontia chrysosporium
1.14.14.1 W129F/L324G the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (15%) toward phenanthrene Phanerodontia chrysosporium
1.14.14.1 W129L/L324F the mutant shows greater oxidation of phenanthrene and pyrene compared with the wild type enzyme Phanerodontia chrysosporium
1.14.14.1 W129L/L324G the mutant shows complete loss of activity toward both phenanthrene and pyrene Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Phanerodontia chrysosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 phenanthrene + [reduced NADPH-hemoprotein reductase] + O2
-
Phanerodontia chrysosporium ?
-
?
1.14.14.1 pyrene + [reduced NADPH-hemoprotein reductase] + O2
-
Phanerodontia chrysosporium ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 CYP5136A3
-
Phanerodontia chrysosporium
1.14.14.1 cytochrome P450 monooxygenase
-
Phanerodontia chrysosporium