EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.5 | expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.5 | D254R/D256R | complete loss of activity | Thermus thermophilus |
5.3.1.5 | D256R | the mutant shows an increase in the specificity on D-lyxose, L-arabinose and D-mannose | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.5 | Co2+ | about 58% residual activity at 1 mM | Thermus thermophilus | |
5.3.1.5 | Cu2+ | less than 10% residual activity at 1 mM | Thermus thermophilus | |
5.3.1.5 | Mg2+ | about 35% residual activity at 1 mM | Thermus thermophilus | |
5.3.1.5 | Ni2+ | less than 5% residual activity at 1 mM | Thermus thermophilus | |
5.3.1.5 | Zn2+ | complete inhibition at 1 mM | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 138 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 185 | - |
D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 215 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 605 | - |
D-xylose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 1005 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 1450 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.5 | Mn2+ | maximal activity with 1 mM Mn2+ | Thermus thermophilus | |
5.3.1.5 | additional information | not stimulated by K+, Ni2+ and Zn2+ | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 44000 | - |
x * 44000, SDS-PAGE | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.5 | D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
r | |
5.3.1.5 | D-Xylose | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
D-Xylulose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.5 | Thermus thermophilus | P26997 | - |
- |
5.3.1.5 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P26997 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.5 | Ni-NTA column chromatography | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 132 | - |
wild type enzyme, with L-arabinose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
5.3.1.5 | 273 | - |
wild type enzyme, with D-mannose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
5.3.1.5 | 330 | - |
mutant enzyme D256R, with L-arabinose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
5.3.1.5 | 380 | - |
wild type enzyme, with D-lyxose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
5.3.1.5 | 682 | - |
mutant enzyme D256R, with D-mannose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
5.3.1.5 | 950 | - |
mutant enzyme D256R, with D-lyxose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.5 | D-fructose | - |
Thermus thermophilus | D-mannose | - |
r | |
5.3.1.5 | D-Glucose | - |
Thermus thermophilus | D-Fructose | - |
r | |
5.3.1.5 | D-Glucose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Fructose | - |
r | |
5.3.1.5 | D-Lyxose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
5.3.1.5 | D-Lyxose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Xylulose | - |
r | |
5.3.1.5 | D-Mannose | - |
Thermus thermophilus | D-Fructose | - |
r | |
5.3.1.5 | D-Mannose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Fructose | - |
r | |
5.3.1.5 | D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
5.3.1.5 | D-Xylose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Xylulose | - |
r | |
5.3.1.5 | L-arabinose | - |
Thermus thermophilus | L-ribulose | - |
r | |
5.3.1.5 | L-arabinose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-ribulose | - |
r | |
5.3.1.5 | L-ribulose | - |
Thermus thermophilus | L-ribose | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.5 | ? | x * 44000, SDS-PAGE | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.5 | D-xylose: ketol-isomerase | - |
Thermus thermophilus |
5.3.1.5 | XYLA | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 85 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 55 | 95 | about 52% activity at 55°C, about 65% activity at 60°C, about 75% activity at 65°C, about 78% activity at 70°C, about 85% activity at 75°C, about 95% activity at 80°C, about 78% activity at 90°C, and about 52% activity at 95°C | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 2 | 8 | D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 6.5 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 13 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 18 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 25 | - |
D-Lyxose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 38 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 7 | - |
- |
Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 5 | 9 | about 40% activity at pH 5.0, about 60% activity at pH 5.5, about 88% activity at pH 6.0 and 8.0, about 92% activity at pH 6.5 and 7.5, about 80% activity at pH 8.5, about 75% activity at pH 9.0 | Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 0.008 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 0.017 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 0.041 | - |
D-Lyxose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 0.09 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 0.15 | - |
D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
5.3.1.5 | 0.17 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus |