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Literature summary extracted from

  • De Marcos Lousa, C.; van Roermund, C.W.; Postis, V.L.; Dietrich, D.; Kerr, I.D.; Wanders, R.J.; Baldwin, S.A.; Baker, A.; Theodoulou, F.L.
    Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids (2013), Proc. Natl. Acad. Sci. USA, 110, 1279-1284.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.20 ATP
-
Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 recombinantly expressed in insect cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 S810N mutant is defective in fatty acid degradation in vivo, retains ATPase activity and has strongly reduced thioesterase activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.0015
-
stearoyl-CoA pH 8, temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.20 membrane
-
Arabidopsis thaliana 16020
-
6.2.1.3 peroxisome
-
Arabidopsis thaliana 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 150000
-
SDS-PAGE Arabidopsis thaliana
6.2.1.3 75000
-
2 * 75000, SDS-PAGE Arabidopsis thaliana
6.2.1.3 150000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 ATP + arachidonate + CoA Arabidopsis thaliana
-
AMP + diphosphate + arachidonoyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Arabidopsis thaliana
-
-
-
6.2.1.3 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 myristoyl-CoA + H2O lower activity with myristoyl-CoA compared to stearoyl-CoA Arabidopsis thaliana CoA + myristate
-
?
3.1.2.20 stearoyl-CoA + H2O
-
Arabidopsis thaliana CoA + stearate
-
?
6.2.1.3 ATP + arachidonate + CoA
-
Arabidopsis thaliana AMP + diphosphate + arachidonoyl-CoA
-
?
6.2.1.3 ATP + octanoate + CoA
-
Arabidopsis thaliana AMP + diphosphate + octanoyl-CoA
-
?
6.2.1.3 ATP + oleate + CoA isoform LACS7 has a much higher ratio of oleoyl-CoA to octanoyl-CoA synthetase activity compared to isoform LCS6 Arabidopsis thaliana AMP + diphosphate + oleoyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 homodimer 2 * 75000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 acyl-CoA thioesterase
-
Arabidopsis thaliana
3.1.2.20 comatose
-
Arabidopsis thaliana
3.1.2.20 CTS
-
Arabidopsis thaliana
6.2.1.3 LACS6 isoform Arabidopsis thaliana
6.2.1.3 LACS7 isoform Arabidopsis thaliana
6.2.1.3 long chain acyl-CoA synthetase
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 5.7
-
stearoyl-CoA pH 8, temperature not specified in the publication Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
Arabidopsis thaliana