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Literature summary extracted from

  • Ling, J.; Peterson, K.M.; Simonovic, I.; Cho, C.; Soell, D.; Simonovic, M.
    Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment (2012), Proc. Natl. Acad. Sci. USA, 109, 3281-3286.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.3 expressed in Escherichia coli BL21-Codon plus cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.3 D423A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 D437A the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 E401A the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 K408A the mutant shows about wild type catalytic efficiency for tRNAThr1 Saccharomyces cerevisiae
6.1.1.3 K440A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 N356A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 N359A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 N400A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 N432A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
6.1.1.3 N432A the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well Saccharomyces cerevisiae
6.1.1.3 Q362A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae
6.1.1.3 R434A the mutant shows weaker binding for tRNAThr1 only Saccharomyces cerevisiae
6.1.1.3 R439A the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well Saccharomyces cerevisiae
6.1.1.3 S409E the mutant shows about wild type catalytic efficiency for tRNAThr1 Saccharomyces cerevisiae
6.1.1.3 T357A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae
6.1.1.3 Y405A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.3 0.00013
-
 tRNA1Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.0002
-
 tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00024
-
 tRNA1Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00024
-
 tRNA1Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00027
-
 tRNA2Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00028
-
 tRNA1Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00029
-
 tRNA1Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.0003
-
 tRNA1Thr mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.0003
-
 tRNA1Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00031
-
 tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00031
-
 tRNA1Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00037
-
 tRNA2Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00044
-
 tRNA2Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00044
-
 tRNA2Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00046
-
 tRNA1Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00047
-
 tRNA1Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00048
-
 tRNA1Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00049
-
 tRNA1Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00049
-
 tRNA2Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00051
-
 tRNA2Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00059
-
 tRNA2Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00059
-
 tRNA1Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00064
-
 tRNA2Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00069
-
 tRNA2Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00078
-
 tRNA2Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00083
-
 tRNA1Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00083
-
 tRNA2Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00094
-
 tRNA2Thr mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00095
-
 tRNA1Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00123
-
 tRNA2Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.00139
-
 tRNA2Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.0014
-
 tRNA2Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.3 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Zn2+ contains a Zn2+ ion Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNA1Thr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-threonyl-tRNA1Thr
-
 ?
6.1.1.3 ATP + L-threonine + tRNA2Thr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-threonyl-tRNA2Thr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.3
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNA1Thr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNA1Thr
-
 ?
6.1.1.3 ATP + L-threonine + tRNA2Thr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNA2Thr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Mst1
-
 Saccharomyces cerevisiae
6.1.1.3 Threonyl-tRNA synthetase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.3 0.01
-
 tRNA2Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.017
-
 tRNA2Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.03
-
 tRNA2Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.033
-
 tRNA1Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.038
-
 tRNA2Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.04
-
 tRNA2Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.043
-
 tRNA2Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.045
-
 tRNA2Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.045
-
 tRNA2Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.05
-
 tRNA1Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.05
-
 tRNA1Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.05
-
 tRNA1Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.05
-
 tRNA1Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.053
-
 tRNA1Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.053
-
 tRNA2Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.055
-
 tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.058
-
 tRNA2Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.06
-
 tRNA1Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.06
-
 tRNA2Thr mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.067
-
 tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.067
-
 tRNA2Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.073
-
 tRNA1Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.08
-
 tRNA1Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.082
-
 tRNA1Thr mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.087
-
 tRNA1Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.088
-
 tRNA2Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.093
-
 tRNA1Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.1
-
 tRNA2Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.103
-
 tRNA2Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.112
-
 tRNA1Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.117
-
 tRNA1Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
6.1.1.3 0.118
-
 tRNA1Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
 Saccharomyces cerevisiae