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Literature summary extracted from

  • Lang, M.; Braun, C.L.; Kanost, M.R.; Gorman, M.J.
    Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster (2012), Proc. Natl. Acad. Sci. USA, 109, 13337-13342.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.3.1 recombinantly expressed in Sf9 cells using the baculovirus expression system. Recombinant MCO1 is expressed without the von Willebrand factor domains and transmembrane segment, and Arg454 is mutated to alanine to reduce proteolytic cleavage Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.16.3.1 R454A Arg454 is mutated to Ala in order to reduce proteolytic cleavage Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.1 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.3.1 using concanavalin-A-Sepharose and Q-sepharose Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.3.1 malpighian tubule
-
 Drosophila melanogaster
-
1.16.3.1 midgut basal surface of midgut Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.3.1 additional information
-
 MCO1 has ferroxidase activity Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
1.16.3.1 MCO1
-
 Drosophila melanogaster
1.16.3.1 multicopper oxidase-1
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.3.1 7
-
 assay at Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.16.3.1 6 8 highest activity Drosophila melanogaster

General Information

EC Number General Information Comment Organism
1.16.3.1 malfunction knockdown of MCO1 is correlated with increased longevity on high-iron food and decreased iron accumulation Drosophila melanogaster