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Literature summary extracted from

  • O'Donoghue, P.; Sheppard, K.; Nureki, O.; Soell, D.
    Rational design of an evolutionary precursor of glutaminyl-tRNA synthetase (2011), Proc. Natl. Acad. Sci. USA, 108, 20485-20490.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.17 expressed in Escherichia coli BL21(DE3) cells Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.7 additional information activity of the engineered enzyme variants indicates that the acceptor stem loop is the principle discrimination element because insertion of this loop alone enhances the specificity of the archaeal enzyme toward tRNAGln2 Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 0.00133
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 0.00361
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 0.00539
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 additional information
-
 additional information aminoacylation kinetics of enzyme variants Methanothermobacter thermautotrophicus
6.3.5.7 0.00133
-
 tRNAGln2(CUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 0.00361
-
 tRNAGlu pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 0.00539
-
 tRNAGln1(UUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln(CUG) Methanothermobacter thermautotrophicus the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGln(UUG) Methanothermobacter thermautotrophicus the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Methanothermobacter thermautotrophicus
-
-
-
6.3.5.7 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.7
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln(CUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGln(UUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.3.5.7 ATP + L-glutamate + tRNAGln1(UUG) the enzyme is active toward the two tRNAGln isoacceptors, but with a significant catalytic preference for tRNAGln2(CUG). The less active tRNAGln1(UUG) responds to the less common CAA codon for Gln Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln1(UUG)
-
 ?
6.3.5.7 ATP + L-glutamate + tRNAGln2(CUG) the enzyme is active toward the two tRNAGln isoacceptors, but with a significant catalytic preference for tRNAGln2(CUG). The less active tRNAGln1(UUG) responds to the less common CAA codon for Gln. The wild-type enzyme shows a 24fold catalytic preference for tRNAGln2 over tRNAGlu Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGln2(CUG)
-
 ?
6.3.5.7 ATP + L-glutamate + tRNAGlu
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
 Methanothermobacter thermautotrophicus
6.1.1.17 Glutamyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus
6.3.5.7 GluRS
-
 Methanothermobacter thermautotrophicus
6.3.5.7 nondiscriminating GluRS
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.7 37
-
 assay at Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.17 0.006
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 0.04
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 0.041
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 additional information
-
 additional information aminoacylation kinetics of enzyme variants Methanothermobacter thermautotrophicus
6.3.5.7 0.006
-
 tRNAGln1(UUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 0.04
-
 tRNAGlu pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 0.41
-
 tRNAGln2(CUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.7 7.2
-
 assay at Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
 Methanothermobacter thermautotrophicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.17 1
-
 tRNAGln(UUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 10
-
 tRNAGlu wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.1.1.17 310
-
 tRNAGln(CUG) wild type enzyme, pH and temperature not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 additional information
-
 additional information aminoacylation kinetics of enzyme variants Methanothermobacter thermautotrophicus
6.3.5.7 1.1
-
 tRNAGln1(UUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 11
-
 tRNAGlu pH 7.2, 37°C Methanothermobacter thermautotrophicus
6.3.5.7 308
-
 tRNAGln2(CUG) pH 7.2, 37°C Methanothermobacter thermautotrophicus