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Literature summary extracted from

  • Lu, X.; Zhang, F.; Shen, Q.; Jiang, W.; Pan, Q.; Lv, Z.; Yan, T.; Fu, X.; Wang, Y.; Qian, H.; Tang, K.
    Overexpression of allene oxide cyclase improves the biosynthesis of artemisinin in Artemisia annua L (2014), PLoS ONE, 9, e91741.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.6 cloning of the promoter of AaAOC, the key gene of jasmonate biosynthetic pathway, expression analysis, AaAOC gene overexpression under control of the 35S promoter in Artemisia annua leading to an increase of 1.6 to 5.2fold in enzyme expression and 2 to 4.7fold increase in jasmonate levels, as well as to a significant increase of contents of artemisinin, dihydroartemisinic acid and artemisinic acid, expression analysis by RT-Q-PCR Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.6 (9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate Artemisia annua
-
 (15Z)-12-oxophyto-10,15-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.6 Artemisia annua
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.99.6 flower bud
-
 Artemisia annua
-
5.3.99.6 leaf
-
 Artemisia annua
-
5.3.99.6 additional information ubiquitous expression, expression pattern analysis, overview Artemisia annua
-
5.3.99.6 root
-
 Artemisia annua
-
5.3.99.6 stem
-
 Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.6 (9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
-
 Artemisia annua (15Z)-12-oxophyto-10,15-dienoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.6 AaAOC
-
 Artemisia annua
5.3.99.6 Allene oxide cyclase
-
 Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.6 heme
-
 Artemisia annua

General Information

EC Number General Information Comment Organism
5.3.99.6 metabolism key enzyme of the jasmonate biosynthetic pathway catalysing the formation of (9S,13S)-12-oxo-phytodienoic acid and establishes the stereochemical configuration of naturally occurring jasmonate. Spontaneous hydrolysis of the allene oxide also yields (9S,13S)-12-oxo-phytodienoic acid, although in its racemic form Artemisia annua
5.3.99.6 additional information contents of artemisinin, dihydroartemisinic acid and artemisinic acid, as well as jasmonate levels and expression levels of farnesyl diphosphate synthase, cytochrome P450 dependent hydroxylase, and double bond reductase 2, are increased significantly in AaAOC overexpression Artemisia annua plants Artemisia annua