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Literature summary extracted from

  • Nakano, T.; Ohki, I.; Yokota, A.; Ashida, H.
    MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila (2013), PLoS ONE, 8, e67385.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.109 gene mtnBD, identification of the domains responsible for catalyzing the four reactions from the enzyme, overview. Recombinant expression of His-tagged proteins of full-length enzyme and the MtnB domain in Escherichia coli BL21(DE3) Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.109 S-methyl-5-thio-D-ribulose 1-phosphate Tetrahymena thermophila activity of the MtnB domain 5-(methylthio)-2,3-dioxopentyl phosphate + H2O
-
 ?
5.3.2.5 5-(methylthio)-2,3-dioxopentyl phosphate Tetrahymena thermophila
-
 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.109 Tetrahymena thermophila I7MAL0 gene mtnBD
-
5.3.2.5 Tetrahymena thermophila
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.109 S-methyl-5-thio-D-ribulose 1-phosphate activity of the MtnB domain Tetrahymena thermophila 5-(methylthio)-2,3-dioxopentyl phosphate + H2O
-
 ?
5.3.2.5 5-(methylthio)-2,3-dioxopentyl phosphate
-
 Tetrahymena thermophila 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.109 MtnBD
-
 Tetrahymena thermophila
4.2.1.109 MTRu-1-P dehydratase/enolase
-
 Tetrahymena thermophila
5.3.2.5 DKMTP-1-P enolase
-
 Tetrahymena thermophila
5.3.2.5 MtnBD
-
 Tetrahymena thermophila
5.3.2.5 MtnW
-
 Tetrahymena thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.109 35
-
 assay at Tetrahymena thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.109 8
-
 assay at Tetrahymena thermophila

General Information

EC Number General Information Comment Organism
4.2.1.109 evolution the MtnB domain of Tetrahymena thermophila MtnBD acquired the new function to catalyze both the dehydratase and enolase reactions through evolutionary gene mutations, rather than fusion of MSP genes Tetrahymena thermophila
4.2.1.109 metabolism the MtnBD fusion enzyme in Tetrahymena thermophila catalyzes two of the sequential reactions from the dehydratase to dioxygenase steps in the methionine salvage pathway: 5-methylthioribulose-1-phosphate is converted to 2-oxo-4-methylthiobutyrate, the methionine precursor, by four steps, namely dehydratase, enolase, phosphatase, and dioxygenase reactions. The MtnB domain catalyzes dehydration and enolization and the MtnD domain catalyzes dioxygenation, the phosphatase is distinct from the enzyme. Methionine salvage pathway, overview Tetrahymena thermophila
4.2.1.109 additional information identification of the domains responsible for catalyzing the four reactions from the enzyme, overview Tetrahymena thermophila