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Literature summary extracted from

  • Moreno, J.C.; Pizarro, L.; Fuentes, P.; Handford, M.; Cifuentes, V.; Stange, C.
    Levels of lycopene beta-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota (2013), PLoS ONE, 8, e58144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.19 gene DcLcyb1, quantitative RT-PCR expression analysis, overexpression and heterologous complementation in enzyme-deficient Escherichia coli, transgenic expression of GFP-tagged enzyme in Nicotiana tabacum plants under control of ad35SCaMV promoter Daucus carota

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.19 additional information posttranscriptional gene silencing of gene DcLcyb1 in carrot affects carotenoid levels and endogenous carotenoid gene expression, overview Daucus carota

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.5.1.19 chloroplast subcellular localization study, overview Daucus carota 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.19 delta-carotene Daucus carota
-
 alpha-carotene
-
 ?
5.5.1.19 gamma-carotene Daucus carota
-
 beta-carotene
-
 ?
5.5.1.19 lycopene Daucus carota
-
 gamma-carotene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.19 Daucus carota Q2VEX7 subsp. sativus, cv. Nantaise, gene DcLcyb1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.19 leaf
-
 Daucus carota
-
5.5.1.19 additional information the expression level is higher in the aerial organs throughout Daucus carota development, quantitative RT-PCR expression analysis Daucus carota
-
5.5.1.19 root storage root Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.19 delta-carotene
-
 Daucus carota alpha-carotene
-
 ?
5.5.1.19 gamma-carotene
-
 Daucus carota beta-carotene
-
 ?
5.5.1.19 lycopene
-
 Daucus carota gamma-carotene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.19 DcLCYB1
-
 Daucus carota
5.5.1.19 LCYb
-
 Daucus carota
5.5.1.19 lycopene beta-cyclase 1
-
 Daucus carota

General Information

EC Number General Information Comment Organism
5.5.1.19 malfunction transgenic plants with higher or reduced levels of DcLcyb1 have incremented or reduced levels of chlorophyll, total carotenoids and beta-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes Daucus carota
5.5.1.19 additional information DcLCYB1 has conserved motifs related to lycopene beta-cyclases such as a cyclase motif I and II, a LCY specific motif, a conserved region beta-LCY, a di-nucleotide binding site, a charged region and the beta-LCY motif, beta-LCY CAD region, domains described as essential for lycopene beta-cyclase activity Daucus carota
5.5.1.19 physiological function lycopene beta-cyclase is a key enzyme involved directly in the synthesis of alpha-carotene and beta-carotene through the cyclization of lycopene. The enzyme does not possess an organ specific function and modulates carotenoid gene expression and accumulation in carrot leaves and storage roots. DcLcyb1 participates in the regulation of endogenous DcPsy1, DcPsy2 and DcLcyb2 carotenogenic genes Daucus carota