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Literature summary extracted from

  • Alvarez-Rueda, N.; Fleury, A.; Morio, F.; Pagniez, F.; Gastinel, L.; Le Pape, P.
    Amino acid substitutions at the major insertion loop of Candida albicans sterol 14alpha-demethylase are involved in fluconazole resistance (2011), PLoS ONE, 6, e21239.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expressed in Escherichia coli TOP10F cells and in Pichia pastoris cells Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.154 K143R the mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans
1.14.14.154 V456I the mutation leads to an 8fold increase in fluconazole minimal inhibitory concentrations of Pichia pastoris mutants compared to the wild type controls. The mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans
1.14.14.154 Y447H the mutation leads to an 8fold increase in fluconazole minimal inhibitory concentrations of Pichia pastoris mutants compared to the wild type controls. The mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 fluconazole
-
 Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 56000
-
 x * 56000, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Candida albicans
-
 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Candida albicans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida albicans 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.154 ? x * 56000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 14alpha-demethylase
-
 Candida albicans
1.14.14.154 CYP51
-
 Candida albicans
1.14.14.154 Erg11p
-
 Candida albicans