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Literature summary extracted from

  • Garcia-Torres, I.; Cabrera, N.; Torres-Larios, A.; Rodriguez-Bolanos, M.; Diaz-Mazariegos, S.; Gomez-Puyou, A.; Perez-Montfort, R.
    Identification of amino acids that account for long-range interactions in two triosephosphate isomerases from pathogenic trypanosomes (2011), PLoS ONE, 6, e18791.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expressed in Escherichia coli (DE3)pLysS cells Trypanosoma brucei
5.3.1.1 expressed in Escherichia coli (DE3)pLysS cells Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 4,4'-Dithiopyridine
-
 Trypanosoma brucei
5.3.1.1 4,4'-Dithiopyridine
-
 Trypanosoma cruzi
5.3.1.1 5,5'-dithiobis(2-nitrobenzoate)
-
 Trypanosoma brucei
5.3.1.1 5,5'-dithiobis(2-nitrobenzoate)
-
 Trypanosoma cruzi
5.3.1.1 methylmethane thiosulfonate 60% residual activity at 0.1 mM Trypanosoma brucei
5.3.1.1 methylmethane thiosulfonate almost complete inhibition at 0.1 mM Trypanosoma cruzi
5.3.1.1 N-ethylmaleimide
-
 Trypanosoma brucei
5.3.1.1 N-ethylmaleimide
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.43
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma cruzi
5.3.1.1 0.45
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate Trypanosoma brucei
-
 Glycerone phosphate
-
 r
5.3.1.1 D-Glyceraldehyde 3-phosphate Trypanosoma cruzi
-
 Glycerone phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Trypanosoma brucei
-
-
-
5.3.1.1 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography Trypanosoma brucei
5.3.1.1 ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Trypanosoma brucei Glycerone phosphate
-
 r
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Trypanosoma cruzi Glycerone phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TIM
-
 Trypanosoma brucei
5.3.1.1 TIM
-
 Trypanosoma cruzi
5.3.1.1 Triosephosphate isomerase
-
 Trypanosoma brucei
5.3.1.1 Triosephosphate isomerase
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 4500
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma cruzi
5.3.1.1 5167
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.1 10500
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma cruzi
5.3.1.1 11500
-
 D-glyceraldehyde 3-phosphate in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C Trypanosoma brucei