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Literature summary extracted from

  • Rao, L.; Zhao, X.; Pan, F.; Li, Y.; Xue, Y.; Ma, Y.; Lu, J.R.
    Solution behavior and activity of a halophilic esterase under high salt concentration (2009), PLoS One, 4, e6980.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 overexpressed from Escherichia coli BL21 Haloarcula marismortui

General Stability

EC Number General Stability Organism
3.1.1.1 influence of NaCl on the stability of the enzyme is highly temperature dependent. At 22°C, the enzyme retains its highest activity when incubated at the high salt concentration of 3.4 M NaCl for over 100 hours. In contrast, the enzyme steadily loses its activity without salt, with most of its activity being lost within 50 h. As the incubation temperature is raised to 37°C, the activities under both solution conditions show a clear sign of decline, although the presence of 3.4 M NaCl sustains the activity more effectively. When the incubation temperature reaches 55°C, the rates of activity loss are significantly increased with and without the addition of 3.4 M NaCl. With further increase in temperature to 60°C the trend of acceleration of activity loss continues and salt addition again accelerates the rate of denaturation, confirming that whilst salt addition helps stabilize the enzyme below 50°C, the opposite effect is produced above it Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 KCl optimal concentration: 3.0 M Haloarcula marismortui
3.1.1.1 NaCl optimal concentration: 3.4 M Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 50000
-
 x * 50000, SDS-PAGE Haloarcula marismortui

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Haloarcula marismortui Q5V5N6
-
-
3.1.1.1 Haloarcula marismortui DSM 3752 Q5V5N6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Haloarcula marismortui 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Haloarcula marismortui DSM 3752 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O about 60% of the activity with 4-nitrophenyl acetate Haloarcula marismortui 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O about 60% of the activity with 4-nitrophenyl acetate Haloarcula marismortui DSM 3752 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O about 30% of the activity with 4-nitrophenyl acetate Haloarcula marismortui 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O about 30% of the activity with 4-nitrophenyl acetate Haloarcula marismortui DSM 3752 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O about 5% of the activity with 4-nitrophenyl acetate Haloarcula marismortui 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O about 5% of the activity with 4-nitrophenyl acetate Haloarcula marismortui DSM 3752 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl propanoate + H2O about 95% of the activity with 4-nitrophenyl acetate Haloarcula marismortui 4-nitrophenol + propanoate
-
 ?
3.1.1.1 4-nitrophenyl propanoate + H2O about 95% of the activity with 4-nitrophenyl acetate Haloarcula marismortui DSM 3752 4-nitrophenol + propanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 50000, SDS-PAGE Haloarcula marismortui

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 LipC
-
 Haloarcula marismortui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 45
-
-
 Haloarcula marismortui

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 40 55 40°C: about 65% of maximal activity, 55°C: about 75% of maximal activity Haloarcula marismortui

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50
-
 whilst salt addition helps stabilize the enzyme below 50°C, the opposite effect is produced above it Haloarcula marismortui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9.5
-
-
 Haloarcula marismortui

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 8 10 pH 8.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Haloarcula marismortui