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Literature summary extracted from

  • Lakhal-Naouar, I.; Jardim, A.; Strasser, R.; Luo, S.; Kozakai, Y.; Nakhasi, H.L.; Duncan, R.C.
    Leishmania donovani argininosuccinate synthase is an active enzyme associated with parasite pathogenesis (2012), PLoS Negl. Trop. Dis., 6, e1849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.5 expressed in Escherichia coli BL21 (DE3) pLysS cells Leishmania donovani

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.5 G128S inactive Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.5 N-methylaspartic acid at 1 mM methyl-DL-aspartic acid the enzyme activity is reduced by about 60%, while at 5 mM the catalytic activity is completely abolished Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.5 cytosol the majority of the enzyme is in the cytosol Leishmania donovani 5829
-
6.3.4.5 vesicle only a small proportion of the total cellular enzyme is localized in vesicles Leishmania donovani 31982
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 46000
-
x * 46000, calculated from amino acid sequence Leishmania donovani
6.3.4.5 49000
-
x * 49000, recombinant enzyme, SDS-PAGE Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate Leishmania donovani
-
AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Leishmania donovani H9BG20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5 Ni-NTA agarose column chromatography Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 amastigote more abundantly expressed in amastigotes than in promastigotes Leishmania donovani
-
6.3.4.5 promastigote
-
Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate
-
Leishmania donovani AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.4.5 ? x * 49000, recombinant enzyme, SDS-PAGE Leishmania donovani
6.3.4.5 ? x * 46000, calculated from amino acid sequence Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
6.3.4.5 ASS
-
Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.5 ATP
-
Leishmania donovani

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.4.5 Leishmania donovani calculated from amino acid sequence
-
5.8

General Information

EC Number General Information Comment Organism
6.3.4.5 physiological function the enzyme is essential for parasite survival and growth in the mammalian host Leishmania donovani