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Literature summary extracted from

  • Besseau, S.; Kellner, F.; Lanoue, A.; Thamm, A.M.; Salim, V.; Schneider, B.; Geu-Flores, F.; Hoefer, R.; Guirimand, G.; Guihur, A.; Oudin, A.; Glevarec, G.; Foureau, E.; Papon, N.; Clastre, M.; Giglioli-Guivarch, N.; St-Pierre, B.; Werck-Reichhart, D.; Burlat, V.; De Luca, V.; OConnor, S.E.; Cou, C.o.u.r.
    A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ-dependent manner in Catharanthus roseus (2013), Plant Physiol., 163, 1792-1803.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.103 expressed in Saccharomyces cerevisiae WAT11 cells Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.103 0.00007
-
 tabersonine isoform T16H2, at pH 8.0 and 30°C Catharanthus roseus
1.14.14.103 0.00035
-
 tabersonine isoform T16H1, at pH 8.0 and 30°C Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.103 endoplasmic reticulum CYP71D351 (isoform T16H2) Catharanthus roseus 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.103 2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus low activity 2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.103 2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus low activity 2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.103 additional information Catharanthus roseus no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine ?
-
 ?
1.14.14.103 tabersonine + NADPH + H+ + O2 Catharanthus roseus highest activity 16-hydroxytabersonine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.103 Catharanthus roseus C0KYN4 CYP71D12; cultivar Pacifica Pink
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.103 flower isoform T16H1, CYP71D351 Catharanthus roseus
-
1.14.14.103 leaf epidermis highest expression of isoform T16H2, CYP71D12 in young leaves Catharanthus roseus
-
1.14.14.103 additional information CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds Catharanthus roseus
-
1.14.14.103 additional information CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.103 2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2 low activity Catharanthus roseus 2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.103 2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2 low activity Catharanthus roseus 2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.103 additional information no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine Catharanthus roseus ?
-
 ?
1.14.14.103 tabersonine + NADPH + H+ + O2 highest activity Catharanthus roseus 16-hydroxytabersonine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.103 CYP71D12 isoform T16H2 Catharanthus roseus
1.14.14.103 CYP71D351 isoform T16H1 Catharanthus roseus
1.14.14.103 T16H1 isoform Catharanthus roseus
1.14.14.103 T16H2 isoform Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.14.14.103 metabolism CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers Catharanthus roseus
1.14.14.103 metabolism CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves Catharanthus roseus