EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.70 | expressed in Escherichia coli strain C41 | Oryza sativa |
1.14.14.86 | expressed in Escherichia coli BL21(DE3) cells | Oryza sativa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.70 | 0.002 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 0.0023 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 0.007 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.70 | ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa | - |
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.70 | ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa | - |
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.70 | ent-sandaracopimaradiene + NADPH + H+ + O2 | Oryza sativa | - |
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O | - |
? | |
1.14.14.86 | ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa | - |
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.70 | Oryza sativa | - |
- |
- |
1.14.14.86 | Oryza sativa | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.70 | ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | 3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.70 | ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | 3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.70 | ent-sandaracopimaradiene + NADPH + H+ + O2 | - |
Oryza sativa | ent-sandaracopimaradien-3beta-ol + NADP+ + H2O | - |
? | |
1.14.14.86 | ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | (-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.70 | CYP701A8 | - |
Oryza sativa |
1.14.14.70 | KOL4 | - |
Oryza sativa |
1.14.14.86 | CYP701A6 | - |
Oryza sativa |
1.14.14.86 | KO2 | - |
Oryza sativa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.70 | 0.013 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 0.017 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 0.022 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.70 | NADPH | - |
Oryza sativa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.70 | 3 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 6 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
1.14.14.70 | 9 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa |