Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.
    CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism (2012), Plant Physiol., 158, 1418-1425.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.70 expressed in Escherichia coli strain C41 Oryza sativa
1.14.14.86 expressed in Escherichia coli BL21(DE3) cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.70 0.002
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 0.0023
-
ent-sandaracopimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 0.007
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.70 ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.70 ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.70 ent-sandaracopimaradiene + NADPH + H+ + O2 Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
?
1.14.14.86 ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.70 Oryza sativa
-
-
-
1.14.14.86 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.70 ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa 3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.70 ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa 3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.70 ent-sandaracopimaradiene + NADPH + H+ + O2
-
Oryza sativa ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
?
1.14.14.86 ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa (-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.70 CYP701A8
-
Oryza sativa
1.14.14.70 KOL4
-
Oryza sativa
1.14.14.86 CYP701A6
-
Oryza sativa
1.14.14.86 KO2
-
Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.70 0.013
-
ent-sandaracopimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 0.017
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 0.022
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.70 NADPH
-
Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.70 3
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 6
-
ent-sandaracopimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.70 9
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa