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Literature summary extracted from

  • Gui, J.; Shen, J.; Li, L.
    Functional characterization of evolutionarily divergent 4-coumarate:coenzyme a ligases in rice (2011), Plant Physiol., 157, 574-586.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 gene Os4CL1, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis Oryza sativa
6.2.1.12 gene Os4CL2, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis Oryza sativa
6.2.1.12 gene Os4CL3, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis Oryza sativa
6.2.1.12 gene Os4CL4, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis Oryza sativa
6.2.1.12 gene Os4CL5, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.0022
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0035
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.0039
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.0046
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.0049
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.0058
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.0069
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.0083
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0094
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0103
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.0109
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.0119
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0157
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.0168
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0217
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0261
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.0276
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0282
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.0293
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0544
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.0589
-
 sinapate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+ required Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Oryza sativa
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Oryza sativa P17814 Os4Cl1; isozyme Os4Cl1
-
6.2.1.12 Oryza sativa Q42982 Os4Cl2; isozyme Os4Cl2
-
6.2.1.12 Oryza sativa Q67W82 Os4Cl4; isozyme Os4Cl4
-
6.2.1.12 Oryza sativa Q6ETN3 Os4Cl3; isozyme Os4Cl3
-
6.2.1.12 Oryza sativa Q6ZAC1 Os4Cl5; isozyme Os4Cl5
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 flower
-
 Oryza sativa
-
6.2.1.12 flower in the lemma, palea, stamens, and pistil Oryza sativa
-
6.2.1.12 leaf
-
 Oryza sativa
-
6.2.1.12 leaf in developing vascular bundle cells as well as in parenchyma cells Oryza sativa
-
6.2.1.12 additional information Os4CL3 is expressed in thickening vascular cells and non-thickening parenchyma cells throughout rice growth, immunohistochemic in situ analysis, quantitative reverse transcription-PCR expression analysis of isozymes during growth and development, overview Oryza sativa
-
6.2.1.12 additional information quantitative reverse transcription-PCR expression analysis of isozymes during growth and development, overview Oryza sativa
-
6.2.1.12 root
-
 Oryza sativa
-
6.2.1.12 root exodermis and epidermis cells Oryza sativa
-
6.2.1.12 stem
-
 Oryza sativa
-
6.2.1.12 stem in epidermis cells and sheath parenchyma cells, in which no lignin is being synthesized Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Oryza sativa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA high activity Oryza sativa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Oryza sativa AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA low activity Oryza sativa AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Oryza sativa AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA low activity Oryza sativa AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Oryza sativa AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA high activity Oryza sativa AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA strong preference for ferulate by isozyme Os4CL2, high activity Oryza sativa AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapate + CoA
-
 Oryza sativa AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 additional information high affinity for 4-coumarate and ferulate by isozyme Os4Cl4 Oryza sativa ?
-
 ?
6.2.1.12 additional information high affinity for 4-coumarate and ferulate by isozyme Os4Cl4, no activity with sinapate Oryza sativa ?
-
 ?
6.2.1.12 additional information no activity with sinapate Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-Coumarate:coenzyme A ligase
-
 Oryza sativa
6.2.1.12 4CL
-
 Oryza sativa
6.2.1.12 Os4Cl1
-
 Oryza sativa
6.2.1.12 Os4Cl2
-
 Oryza sativa
6.2.1.12 Os4Cl3
-
 Oryza sativa
6.2.1.12 Os4Cl4
-
 Oryza sativa
6.2.1.12 Os4Cl5
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 additional information
-
 additional information isozyme Os4CL1 displays a very low turnover rate Oryza sativa
6.2.1.12 0.0061
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0068
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0082
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.0087
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.014
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.017
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.0188
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0213
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.032
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.034
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.036
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.0385
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0395
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.0445
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl2 Oryza sativa
6.2.1.12 0.047
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl4 Oryza sativa
6.2.1.12 0.048
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.053
-
 sinapate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl5 Oryza sativa
6.2.1.12 0.182
-
 cinnamate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.255
-
 caffeate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.285
-
 4-coumarate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.299
-
 ferulate pH 7.5, temperature not specified in the publication, recombinant isozyme Os4Cl3 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.5
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
6.2.1.12 evolution in rice, Os4CL2 belongs to the same clade as type II 4CLs in dicots, while the rest of the 4CLs (Os4CL1/3/4/5) belongs to a separate cluster, named type III, which is distinct from the lignin-associated type I 4CLs found in dicots Oryza sativa
6.2.1.12 malfunction suppression of Os4CL3 expression results in significant lignin reduction, shorter plant growth, and other morphological changes. 4CL-suppressed transgenics also display decreased panicle fertility, which may be attributed to abnormal anther development as a result of disrupted lignin synthesis, isozyme Os4Cl3 suppressed phenotype, overview Oryza sativa
6.2.1.12 metabolism key enzyme in the phenylpropanoid metabolic pathways for monolignol and flavonoid biosynthesis Oryza sativa
6.2.1.12 additional information rice 4CL isoforms display different substrate specificities and catalytic turnover rates Oryza sativa
6.2.1.12 physiological function Os4CL3 may play a role in the synthesis of lignin as well as other phenolic compounds Oryza sativa