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Literature summary extracted from

  • Wang, P.; Li, X.Z.; Cui, H.R.; Feng, Y.G.; Wang, X.Y.
    Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum (2014), Plant Physiol. Biochem., 76, 58-66.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.8 gene PPI, phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of GhPPI-GFP fusion enzyme in onion epidermal cell nuclei from pBI121-GFP using Agrobacterium tumefaciens strain LBA4404 for transfection Gossypium hirsutum

Protein Variants

EC Number Protein Variants Comment Organism
5.2.1.8 C138A site-directed mutagenesis, the mutant shows 93% of wild-type activity Gossypium hirsutum
5.2.1.8 C41A site-directed mutagenesis, the mutant shows 104% of wild-type activity Gossypium hirsutum
5.2.1.8 C51A site-directed mutagenesis, the mutant shows 93% of wild-type activity Gossypium hirsutum
5.2.1.8 C90A site-directed mutagenesis, the mutant shows 35% of wild-type activity Gossypium hirsutum
5.2.1.8 F111A site-directed mutagenesis, the mutant shows 30% of wild-type activity Gossypium hirsutum
5.2.1.8 F111Y site-directed mutagenesis, the mutant shows 20% of wild-type activity Gossypium hirsutum
5.2.1.8 H131A site-directed mutagenesis, the mutant shows 19% of wild-type activity Gossypium hirsutum
5.2.1.8 H134A site-directed mutagenesis, the mutant shows 30% of wild-type activity Gossypium hirsutum
5.2.1.8 H48A site-directed mutagenesis, the mutant shows 7.8% of wild-type activity Gossypium hirsutum
5.2.1.8 M107A site-directed mutagenesis, the mutant shows 73% of wild-type activity Gossypium hirsutum
5.2.1.8 T130A site-directed mutagenesis, the mutant shows 68% of wild-type activity Gossypium hirsutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.2.1.8 nucleus
-
 Gossypium hirsutum 5634
-

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.8 Gossypium hirsutum Q4KNC9
-
-
5.2.1.8 Gossypium hirsutum ZM3 Q4KNC9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.8 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.8 additional information purified recombinant GhPPI accelerates the initial velocity of the cis-trans conversion of peptidyl-prolyl bonds of a tetrapeptide in a GhPPI concentration-dependent manner. Recombinant GhPPI also suppresses protein aggregation under denaturing conditions using guanidine hydrochloride, suggesting an additional chaperone activity Gossypium hirsutum ?
-
 ?
5.2.1.8 additional information purified recombinant GhPPI accelerates the initial velocity of the cis-trans conversion of peptidyl-prolyl bonds of a tetrapeptide in a GhPPI concentration-dependent manner. Recombinant GhPPI also suppresses protein aggregation under denaturing conditions using guanidine hydrochloride, suggesting an additional chaperone activity Gossypium hirsutum ZM3 ?
-
 ?
5.2.1.8 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
 Gossypium hirsutum ?
-
 ?
5.2.1.8 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
 Gossypium hirsutum ZM3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.2.1.8 More three-dimensional structure of GhPPI by homology modeling, molecular docking, structure of the substrate binding site of GhPPI, overview Gossypium hirsutum

Synonyms

EC Number Synonyms Comment Organism
5.2.1.8 parvulin-type peptidyl-prolyl isomerase
-
 Gossypium hirsutum
5.2.1.8 peptidyl-prolyl cis/trans isomerase
-
 Gossypium hirsutum
5.2.1.8 PPIase
-
 Gossypium hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.2.1.8 37
-
 assay at Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.8 8
-
 assay at Gossypium hirsutum

Expression

EC Number Organism Comment Expression
5.2.1.8 Gossypium hirsutum mRNA accumulation of GhPPI is induced by salt stress up

General Information

EC Number General Information Comment Organism
5.2.1.8 evolution the enzyme belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases, PPIases Gossypium hirsutum
5.2.1.8 additional information three-dimensional structure of GhPPI by homology modeling, molecular docking, structure of the substrate binding site of GhPPI, overview Gossypium hirsutum