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Literature summary extracted from
- Identification of a phosphorylation site in cyclobutane pyrimidine dimer photolyase of rice (2013), Plant Physiol. Biochem., 63, 24-29.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.3 | gene phr, expression of wild-type and mutant enzymes from vector pTD1 vector by in vitro translation using a cell-free insect system | Oryza sativa Japonica Group |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | S267A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | S494A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | S504A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | S5A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | S7A | site-directed mutagenesis, the mutant enzyme is not phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | S84A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
| 4.1.99.3 | T115A | site-directed mutagenesis, the mutant enzyme is phosphorylated | Oryza sativa Japonica Group |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.99.3 | chloroplast | contains a relatively high proportion of the phosphorylated CPD photolyase | Oryza sativa Japonica Group | 9507 | - |
| 4.1.99.3 | chloroplast | contains a relatively high proportion of the phosphorylated CPD photolyase | Oryza meridionalis | 9507 | - |
| 4.1.99.3 | mitochondrion | contains a high proportion of unphosphorylated CPD photolyase | Oryza sativa Japonica Group | 5739 | - |
| 4.1.99.3 | mitochondrion | contains a high proportion of unphosphorylated CPD photolyase | Oryza meridionalis | 5739 | - |
| 4.1.99.3 | additional information | phosphorylation of CPD photolyase is involved in the translocation of this enzyme to organelles | Oryza sativa Japonica Group | - |
- |
| 4.1.99.3 | additional information | phosphorylation of CPD photolyase is involved in the translocation of this enzyme to organelles | Oryza meridionalis | - |
- |
| 4.1.99.3 | nucleus | contains a relatively high proportion of the phosphorylated CPD photolyase | Oryza sativa Japonica Group | 5634 | - |
| 4.1.99.3 | nucleus | contains a relatively high proportion of the phosphorylated CPD photolyase | Oryza meridionalis | 5634 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.3 | 54000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE | Hordeum vulgare |
| 4.1.99.3 | 54000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE | Zea mays |
| 4.1.99.3 | 54000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Triticum aestivum |
| 4.1.99.3 | 54000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza sativa Japonica Group |
| 4.1.99.3 | 54000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza meridionalis |
| 4.1.99.3 | 56000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Triticum aestivum |
| 4.1.99.3 | 56000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza sativa Japonica Group |
| 4.1.99.3 | 56000 | - |
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza meridionalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.3 | Hordeum vulgare | F2E786 | - |
- |
| 4.1.99.3 | Oryza meridionalis | - |
- |
- |
| 4.1.99.3 | Oryza meridionalis W1299 | - |
- |
- |
| 4.1.99.3 | Oryza meridionalis W1629 | - |
- |
- |
| 4.1.99.3 | Oryza sativa Japonica Group | Q6F6A2 | subsp. japonica, gene phr | - |
| 4.1.99.3 | Oryza sativa Japonica Group Sasanishiki | Q6F6A2 | subsp. japonica, gene phr | - |
| 4.1.99.3 | Triticum aestivum | - |
- |
- |
| 4.1.99.3 | Zea mays | B4F9R2 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | additional information | no phosphoprotein | Hordeum vulgare |
| 4.1.99.3 | additional information | no phosphoprotein | Zea mays |
| 4.1.99.3 | phosphoprotein | the enzyme is phosphorylated at Ser7. Phosphorylation of CPD photolyase is involved in the translocation of the enzyme to organelles | Oryza meridionalis |
| 4.1.99.3 | phosphoprotein | the enzyme is phosphorylated at Ser7. Residues Ser5, Ser84, Ser 267, Ser494, Ser504, and Thr115 are no phorphorylation sites. Phosphorylation of CPD photolyase is involved in the translocation of the enzyme to organelles | Oryza sativa Japonica Group |
| 4.1.99.3 | phosphoprotein | the enzyme is phosphorylated. Phosphorylation of CPD photolyase is probably involved in the translocation of the enzyme to organelles | Triticum aestivum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.3 | native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis | Triticum aestivum |
| 4.1.99.3 | native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis | Oryza sativa Japonica Group |
| 4.1.99.3 | native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis | Oryza meridionalis |
| 4.1.99.3 | native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis | Hordeum vulgare |
| 4.1.99.3 | native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis | Zea mays |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.99.3 | leaf | - |
Triticum aestivum | - |
| 4.1.99.3 | leaf | - |
Oryza sativa Japonica Group | - |
| 4.1.99.3 | leaf | - |
Oryza meridionalis | - |
| 4.1.99.3 | leaf | - |
Hordeum vulgare | - |
| 4.1.99.3 | leaf | - |
Zea mays | - |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | ? | x * 54000, unphosphorylated enzyme, SDS-PAGE | Hordeum vulgare |
| 4.1.99.3 | ? | x * 54000, unphosphorylated enzyme, SDS-PAGE | Zea mays |
| 4.1.99.3 | ? | x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Triticum aestivum |
| 4.1.99.3 | ? | x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza sativa Japonica Group |
| 4.1.99.3 | ? | x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE | Oryza meridionalis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | CPD photolyase | - |
Triticum aestivum |
| 4.1.99.3 | CPD photolyase | - |
Oryza sativa Japonica Group |
| 4.1.99.3 | CPD photolyase | - |
Oryza meridionalis |
| 4.1.99.3 | CPD photolyase | - |
Hordeum vulgare |
| 4.1.99.3 | CPD photolyase | - |
Zea mays |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Triticum aestivum |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Oryza sativa Japonica Group |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Oryza meridionalis |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Hordeum vulgare |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Zea mays |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | evolution | the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis strains shows several amino acid differences, strain W1299 has the alterations F114L, Q126R, I250M, D258H, F260Y, C305R, V313I, Q367H, Y434F and P495L, and strain W1626 P13S, Q126R and T416P | Oryza sativa Japonica Group |
| 4.1.99.3 | evolution | the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis W1299 strain shows ten amino acid differences, strain W1299 has the alterations: F114L, Q126R, I250M, D258H, F260Y, C305R, V313I, Q367H, Y434F and P495L | Oryza meridionalis |
| 4.1.99.3 | evolution | the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis W1626 strain shows three amino acid differences, strain W1299 has the alterations: P13S, Q126R and T416P | Oryza meridionalis |
| 4.1.99.3 | physiological function | in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity | Triticum aestivum |
| 4.1.99.3 | physiological function | in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity | Oryza sativa Japonica Group |
| 4.1.99.3 | physiological function | in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity | Oryza meridionalis |
| 4.1.99.3 | physiological function | in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity | Hordeum vulgare |
| 4.1.99.3 | physiological function | in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity | Zea mays |
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