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Literature summary extracted from

  • Rastogi, S.; Kumar, R.; Chanotiya, C.S.; Shanker, K.; Gupta, M.M.; Nagegowda, D.A.; Shasany, A.K.
    4-coumarate: CoA ligase partitions metabolites for eugenol biosynthesis (2013), Plant Cell Physiol., 54, 1238-1252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 DNA and amino acid sequence determination and analysis, sequence comparisons Ocimum basilicum
6.2.1.12 DNA and amino acid sequence determination and analysis, sequence comparisons Agastache rugosa
6.2.1.12 DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of OS4CL-GFP-fusion protein in Arabidopsis thaliana protoplast cytosol, expression of N-terminally His6-tagged OS4CL in Escherichia coli Ocimum tenuiflorum

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.12 additional information transient silencing of the OS4CL gene in planta by RNA interference Ocimum tenuiflorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.00166
-
 4-coumarate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 0.00302
-
 ferulate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 0.00494
-
 caffeate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 0.02576
-
 trans-cinnamate pH 7.5, 30°C Ocimum tenuiflorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+ required Ocimum tenuiflorum
6.2.1.12 Mg2+ required Ocimum basilicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 62480
-
 x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE Ocimum tenuiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Ocimum tenuiflorum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA Ocimum basilicum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA Ocimum basilicum CIM SAUMYA
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA Ocimum tenuiflorum CIM AYU
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA Ocimum tenuiflorum
-
 AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA Ocimum tenuiflorum CIM AYU
-
 AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA Ocimum tenuiflorum
-
 AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA Ocimum tenuiflorum CIM AYU
-
 AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + trans-cinnamate + CoA Ocimum tenuiflorum
-
 AMP + diphosphate + trans-cinnamoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Agastache rugosa Q6PQ01
-
-
6.2.1.12 Ocimum basilicum S4WX39 isozyme OB4CL_ctg4
-
6.2.1.12 Ocimum basilicum CIM SAUMYA S4WX39 isozyme OB4CL_ctg4
-
6.2.1.12 Ocimum tenuiflorum T1NXG6 i.e. Ocimum tenuiflorum, isozyme OS4CL
-
6.2.1.12 Ocimum tenuiflorum CIM AYU T1NXG6 i.e. Ocimum tenuiflorum, isozyme OS4CL
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 leaf
-
 Ocimum tenuiflorum
-
6.2.1.12 leaf
-
 Ocimum basilicum
-
6.2.1.12 additional information quantitative reverse transcription-PCR expression analysis at different developmental stages, 2, 4 and 6 weeks after seed germination, overview Ocimum tenuiflorum
-
6.2.1.12 root
-
 Ocimum tenuiflorum
-
6.2.1.12 root
-
 Ocimum basilicum
-
6.2.1.12 stem
-
 Ocimum tenuiflorum
-
6.2.1.12 stem
-
 Ocimum basilicum
-
6.2.1.12 trichome glandular trichome-rich tissue Ocimum tenuiflorum
-
6.2.1.12 trichome glandular trichome-rich tissue Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Ocimum tenuiflorum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Ocimum basilicum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA best substrate Ocimum tenuiflorum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Ocimum basilicum CIM SAUMYA AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Ocimum tenuiflorum CIM AYU AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA best substrate Ocimum tenuiflorum CIM AYU AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Ocimum tenuiflorum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Ocimum tenuiflorum CIM AYU AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Ocimum tenuiflorum AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Ocimum tenuiflorum CIM AYU AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + trans-cinnamate + CoA
-
 Ocimum tenuiflorum AMP + diphosphate + trans-cinnamoyl-CoA
-
 ?
6.2.1.12 additional information no activity with sinapic acid Ocimum tenuiflorum ?
-
 ?
6.2.1.12 additional information no activity with sinapic acid Ocimum tenuiflorum CIM AYU ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE Ocimum tenuiflorum

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate: CoA ligase
-
 Ocimum tenuiflorum
6.2.1.12 4-coumarate: CoA ligase
-
 Ocimum basilicum
6.2.1.12 4-coumarate: CoA ligase
-
 Agastache rugosa
6.2.1.12 4-coumarate:CoA ligase
-
 Ocimum tenuiflorum
6.2.1.12 4-coumarate:CoA ligase
-
 Ocimum basilicum
6.2.1.12 4CL
-
 Ocimum tenuiflorum
6.2.1.12 4CL
-
 Ocimum basilicum
6.2.1.12 Ar4CL
-
 Agastache rugosa
6.2.1.12 OB4CL_ctg4
-
 Ocimum basilicum
6.2.1.12 OS4CL
-
 Ocimum tenuiflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 22
-
 assay at room temperature Ocimum basilicum
6.2.1.12 22 30 assay at Ocimum tenuiflorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 0.44
-
 caffeate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 0.58
-
 trans-cinnamate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 0.7 1 4-coumarate pH 7.5, 30°C Ocimum tenuiflorum
6.2.1.12 1.56
-
 ferulate pH 7.5, 30°C Ocimum tenuiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.5
-
 assay at Ocimum tenuiflorum
6.2.1.12 7.5
-
 assay at Ocimum basilicum

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Ocimum tenuiflorum
6.2.1.12 ATP
-
 Ocimum basilicum

General Information

EC Number General Information Comment Organism
6.2.1.12 evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Ocimum tenuiflorum
6.2.1.12 evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Ocimum basilicum
6.2.1.12 evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Agastache rugosa
6.2.1.12 malfunction a significant reduction in the expression levels is observed for OB4CL_ctg4-related transcripts in suppressed trichome compared with transcripts similar to the other four isoforms, OB4CL_ctg1, 2, 3 and 5 Ocimum basilicum
6.2.1.12 malfunction transient RNAi suppression of OS4CL in leaves causes a reduction in leaf eugenol content and trichome transcript level, with a considerable increase in endogenous 4-coumaric, ferulic, trans-cinnamic and caffeic acids, but no significant effect of OS4CL suppression on the total lignin content Ocimum tenuiflorum
6.2.1.12 metabolism the enzyme is involved in the biosynthetic pathway of eugenol/chavicol synthesis in Ocimum sanctum, overview Ocimum tenuiflorum
6.2.1.12 physiological function metabolite channeling of intermediates towards eugenol by a specific 4-coumarate:CoA ligase and involvement of the enzyme in creation of virtual compartments through substrate utilization and committing metabolites for eugenol biosynthesis at an early stage of the pathway, overview Ocimum tenuiflorum