EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme) | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.3 | 0.00018 | - |
FMNH2 | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
1.14.14.3 | 0.0002 | - |
FMNH2 | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
1.14.14.3 | 0.00113 | - |
decanal | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
1.14.14.3 | 0.00165 | - |
decanal | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.3 | DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + hv | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | luciferase | - |
Vibrio harveyi |
1.14.14.3 | Lux | - |
Vibrio harveyi |