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Literature summary extracted from
- Controversial effect of two methylguanidine-based ionic liquids on firefly luciferase (2012), Photochem. Photobiol. Sci., 11, 828-834.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.7 | [1,1,3,3-tetramethylguanidine][lactate] | increase in concentration of [1,1,3,3-tetramethylguanidine][Lac] up to 0.25 M increases enzyme activity, and at concentrations more than 0.25 M enzyme activity is decreased. Optimum temperature and thermal stability studies show more stability of luciferase only in the presence of [1,1,3,3-tetramethylguanidine][Lac] | Photinus pyralis |  |
| 1.13.12.7 | [1,1,3,3-tetramethylguanidine][propionate] | in the presence of [1,1,3,3-tetramethylguanidine][propionate], enzyme activities are decreased with an increase in ionic liquid concentration | Photinus pyralis | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.12.7 | expressed in Escherichia coli as a His-tagged fusion protein | Photinus pyralis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.7 | 0.01 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, native enzyme | Photinus pyralis | |
| 1.13.12.7 | 0.0125 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis | |
| 1.13.12.7 | 0.0625 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis | |
| 1.13.12.7 | 0.0778 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis | |
| 1.13.12.7 | 0.0778 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis | |
| 1.13.12.7 | 0.111 | - |
ATP | pH 7.8, temperature not specified in the publication, native enzyme | Photinus pyralis | |
| 1.13.12.7 | 0.1125 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis | |
| 1.13.12.7 | 0.2 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis | |
| 1.13.12.7 | 0.7 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis | |
| 1.13.12.7 | 1 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.7 | Photinus pyralis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.7 | using Ni-NTA chromatography | Photinus pyralis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.7 | D-luciferin + ATP + O2 | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.12.7 | luciferase | - |
Photinus pyralis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 25 | - |
in the absence of ionic liquids | Photinus pyralis |
| 1.13.12.7 | 25 | - |
in the presence of [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis |
| 1.13.12.7 | 30 | - |
in the presence of [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 22 | 28 | - |
Photinus pyralis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 20 | - |
after 20 min only 5% activity in the absence of ionic liquids but less than 1% in the presence of [1,1,3,3-tetramethylguanidine][lactate] and [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis |
| 1.13.12.7 | 30 | - |
after 20 min 10% remaining activity, native enzyme | Photinus pyralis |
| 1.13.12.7 | 30 | - |
after 20 min more less than 1% remaining activity in the presence of [1,1,3,3-tetramethylguanidine][propionate] | Photinus pyralis |
| 1.13.12.7 | 30 | - |
after 20 min more than 10% remaining activity in the presence of [1,1,3,3-tetramethylguanidine][lactate] | Photinus pyralis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 7.8 | - |
assay at | Photinus pyralis |
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