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Literature summary extracted from
- Purification and characterization of phosphoenolpyruvate carboxykinase from Raillietina echinobothrida, a cestode parasite of the domestic fowl (2013), Parasitology, 140, 136-146.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.32 | GMP | competitive inhibitor | Raillietina echinobothrida |  |
| 4.1.1.32 | GTP | competitive inhibitor | Raillietina echinobothrida | |
| 4.1.1.32 | IMP | competitive inhibitor | Raillietina echinobothrida | |
| 4.1.1.32 | ITP | competitive inhibitor | Raillietina echinobothrida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.32 | 0.02412 | - |
phosphoenolpyruvate | with IDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.02516 | - |
GDP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.04252 | - |
phosphoenolpyruvate | with GDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.1273 | - |
IDP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 4.76 | - |
CO2 | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.32 | Mn2+ | required, 4 mM used in assay conditions | Raillietina echinobothrida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.32 | 65000 | - |
high pressure liquid chromatography | Raillietina echinobothrida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.32 | GDP + phosphoenolpyruvate + CO2 | Raillietina echinobothrida | highest activity | GTP + oxaloacetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.32 | Raillietina echinobothrida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.32 | ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Blue Sepharose CL 6B affinity chromatography | Raillietina echinobothrida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.32 | -80°C, more than 1 year, the enzyme remains stable | Raillietina echinobothrida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.32 | GDP + phosphoenolpyruvate + CO2 | highest activity | Raillietina echinobothrida | GTP + oxaloacetate | - |
? | |
| 4.1.1.32 | IDP + phosphoenolpyruvate + CO2 | lower activity compared to GDP | Raillietina echinobothrida | ITP + oxaloacetate | - |
? | |
| 4.1.1.32 | additional information | no enzyme activity observed when ADP or CDP is used | Raillietina echinobothrida | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.32 | monomer | 1 * 65000, SDS-PAGE | Raillietina echinobothrida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.32 | PEP carboxykinase | - |
Raillietina echinobothrida |
| 4.1.1.32 | PEPCK | - |
Raillietina echinobothrida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.32 | 7.4 | - |
- |
Raillietina echinobothrida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.32 | 6.6 | 8 | there is a steep rise in the enzyme activity (about 2fold) between the pH 6.6 and pH 7.4, although a sharp decline is observed within a narrow range (pH 7.48.0) | Raillietina echinobothrida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.32 | GDP | - |
Raillietina echinobothrida | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.32 | 0.07642 | - |
GTP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.1864 | - |
GMP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.2264 | - |
ITP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
| 4.1.1.32 | 0.3483 | - |
IMP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
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