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Literature summary extracted from
- Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase (2012), Org. Lett., 14, 5222-5225.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.47 | - |
Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme produng exclusively achilleol A as product | Saccharomyces cerevisiae |
| 5.4.99.7 | Q450H/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol, and protosta-16,24-dien-3beta-ol | Saccharomyces cerevisiae |
| 5.4.99.7 | T384Y | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, parkeol, and 9beta-lanosta-7,24-dien-3beta-ol | Saccharomyces cerevisiae |
| 5.4.99.7 | T384Y/Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and very low amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
| 5.4.99.7 | T384Y/Q450H/V454I | site-directed mutagenesis, the mutant produces exclusively reaction intermediate parkeol but not lanosterol as the sole end product | Saccharomyces cerevisiae |
| 5.4.99.7 | T384Y/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and lower amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
| 5.4.99.7 | V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly lanosterol and a low amount of (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | Saccharomyces cerevisiae |
| 5.4.99.47 | Q450H | nonviable single mutant produces minor amount achilleol A as the sole product | Saccharomyces cerevisiae |
| 5.4.99.47 | Q450H/V454I | mutant produces lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol, protosta-16,24-dien-3beta-ol in a ratio of 75:16:9 | Saccharomyces cerevisiae |
| 5.4.99.47 | T384Y | mutant produces lanosterol, parkeol, 9beta-lanosta-7,24-dien-3 beta-ol, protosta-13(17),24-dien-3beta-ol and protosta-16,24-dien-3beta-ol in the ratio of 63:31.5:3.5:1:1 without compounds achilleol A and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol | Saccharomyces cerevisiae |
| 5.4.99.47 | T384Y/Q450H | mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 1:98:1 | Saccharomyces cerevisiae |
| 5.4.99.47 | T384Y/Q450H/V454I | mutant generates parkeol as the sole product, Vmax/Km 18fold decreased for 3S-2,3-oxidosqualene compared to wild-type | Saccharomyces cerevisiae |
| 5.4.99.47 | T384Y/V454I | mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 10:84:6 | Saccharomyces cerevisiae |
| 5.4.99.47 | V454I | mutant produces poor amounts of lanosterol and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol (ratio 90:10) | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.7 | Saccharomyces cerevisiae | - |
- |
- |
| 5.4.99.47 | Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of wild-type enzyme | Saccharomyces cerevisiae | lanosterol | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H | Saccharomyces cerevisiae | achilleol A | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H/V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol + protosta-16,24-dien-3beta-ol | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutants T384Y, T384Y/Q450H, and T384Y/V454I | Saccharomyces cerevisiae | lanosterol + parkeol + 9beta-lanosta-7,24-dien-3beta-ol | - |
? | |
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant T384Y/Q450H/V454I | Saccharomyces cerevisiae | parkeol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | ERG7 | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | OSC | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |
| 5.4.99.47 | ERG7 | - |
Saccharomyces cerevisiae |
| 5.4.99.47 | Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |
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