Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hu, Q.H.; Huang, Q.; Wang, E.D.
    Crucial role of the C-terminal domain of Mycobacterium tuberculosis leucyl-tRNA synthetase in aminoacylation and editing (2013), Nucleic Acids Res., 41, 1859-1872.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 L949A the mutant shows 9.5% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 L949K the mutant shows 2.6% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 L964A the mutant shows 85% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 L964K the mutant shows 8.6% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 Q915A the mutant shows 99% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 Q915K the mutant shows 54% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 R921A the mutant shows 37% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 R921K the mutant shows 83% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 V910A the mutant shows 90% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 V910P the mutant shows 3.7% activity compared to the wild type enzyme Mycobacterium tuberculosis
6.1.1.4 V910W the mutant shows 93% activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0011
-
tRNALeu mutant enzyme V910A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0011
-
tRNALeu wild type enzyme, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0012
-
tRNALeu mutant enzyme L964A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0013
-
tRNALeu mutant enzyme Q915K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0014
-
tRNALeu mutant enzyme V910W, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0018
-
tRNALeu mutant enzyme R921K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0019
-
tRNALeu mutant enzyme Q915A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0052
-
tRNALeu mutant enzyme R921A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0083
-
tRNALeu mutant enzyme L949A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0092
-
tRNALeu mutant enzyme V910P, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0174
-
tRNALeu mutant enzyme L964K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 0.0273
-
tRNALeu mutant enzyme L949K, at pH 8.2 and 30°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Mg2+ the optimum Mg2+:ATP ratio for aminoacylation is 3:1 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 108000
-
x * 108000, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Mycobacterium tuberculosis
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu Mycobacterium tuberculosis H37Rv
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Mycobacterium tuberculosis
-
-
-
6.1.1.4 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 Ni2+-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
Mycobacterium tuberculosis AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu
-
Mycobacterium tuberculosis H37Rv AMP + diphosphate + L-leucyl-tRNALeu
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 108000, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
Mycobacterium tuberculosis
6.1.1.4 LeuRS
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 30
-
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 2.4
-
tRNALeu mutant enzyme V910P, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 5
-
tRNALeu mutant enzyme L949K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 5
-
tRNALeu mutant enzyme Q915K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 5.6
-
tRNALeu mutant enzyme L949A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 7
-
tRNALeu mutant enzyme V910A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 7.2
-
tRNALeu mutant enzyme L964A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 7.8
-
tRNALeu wild type enzyme, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 9.2
-
tRNALeu mutant enzyme V910W, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 10.6
-
tRNALeu mutant enzyme L964K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 10.6
-
tRNALeu mutant enzyme R921K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 13.3
-
tRNALeu mutant enzyme Q915A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 13.6
-
tRNALeu mutant enzyme R921A, at pH 8.2 and 30°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 8.2
-
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.4 183.2
-
tRNALeu mutant enzyme L949K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 260.9
-
tRNALeu mutant enzyme V910P, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 609.2
-
tRNALeu mutant enzyme L964K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 674.7
-
tRNALeu mutant enzyme L949A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 2615
-
tRNALeu mutant enzyme R921A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 3846
-
tRNALeu mutant enzyme Q915K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 5889
-
tRNALeu mutant enzyme R921K, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 6000
-
tRNALeu mutant enzyme L964A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 6364
-
tRNALeu mutant enzyme V910A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 6571
-
tRNALeu mutant enzyme V910W, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 7000
-
tRNALeu mutant enzyme Q915A, at pH 8.2 and 30°C Mycobacterium tuberculosis
6.1.1.4 7091
-
tRNALeu wild type enzyme, at pH 8.2 and 30°C Mycobacterium tuberculosis