BRENDA - Enzyme Database

Gln-tRNAGln synthesis in a dynamic transamidosome from Helicobacter pylori, where GluRS2 hydrolyzes excess Glu-tRNAGln

Huot, J.L.; Fischer, F.; Corbeil, J.; Madore, E.; Lorber, B.; Diss, G.; Hendrickson, T.L.; Kern, D.; Lapointe, J.; Nucleic Acids Res. 39, 9306-9315 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.5.7
additional information
the presence of 0.0066 mM GluRS2 increases the KM of the enzyme for Glu-tRNAGln nearly 4fold
Helicobacter pylori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.00042
-
Glu-tRNAGln
in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.00097
-
Glu-tRNAGln
in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.00134
-
Glu-tRNAGln
in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
Helicobacter pylori
-
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.7
Helicobacter pylori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.7
DEAE-cellulose column chromatography, Ni-NTA resin column chromatography, and Superdex 200 gel filtration
Helicobacter pylori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
-
728391
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.21
-
Glu-tRNAGln
in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C; in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.23
-
Glu-tRNAGln
in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.3.5.7
ATP
-
Helicobacter pylori
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.5.7
additional information
the presence of 0.0066 mM GluRS2 increases the KM of the enzyme for Glu-tRNAGln nearly 4fold
Helicobacter pylori
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.3.5.7
ATP
-
Helicobacter pylori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.00042
-
Glu-tRNAGln
in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.00097
-
Glu-tRNAGln
in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.00134
-
Glu-tRNAGln
in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
Helicobacter pylori
-
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.7
DEAE-cellulose column chromatography, Ni-NTA resin column chromatography, and Superdex 200 gel filtration
Helicobacter pylori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
-
728391
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.21
-
Glu-tRNAGln
in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C; in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori
6.3.5.7
0.23
-
Glu-tRNAGln
in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C
Helicobacter pylori