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Literature summary extracted from

  • Hwang, K.Y. Chung, J.H.; Kim, S.H.; Han, Y.S.; Cho, Y.
    Structure-based identification of a novel NTPase from Methanococcus jannaschii (1999), Nat. Struct. Biol., 6, 691-696.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.66 expression in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.66 crystals are grown by hanging-drop vapor diffusion method, the crystal structure of the protein is determined at 2.2 A resolution Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.66 0.1
-
 XTP pH 7.6, 80°C Methanocaldococcus jannaschii
3.6.1.66 0.15
-
 ITP pH 7.6, 80°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.66 Mg2+ NTPase activities are most efficient in the presence of either Mg2+ or Mn2+ at 80°C Methanocaldococcus jannaschii
3.6.1.66 Mn2+ NTPase activities are most efficient in the presence of either Mg2+ or Mn2+ at 80°C Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.66 Methanocaldococcus jannaschii Q57679
-
-
3.6.1.66 Methanocaldococcus jannaschii DSM 2661 Q57679
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.66 ITP + H2O catalytic efficiency (kcat/Km) is about 60% compared to the catalytic efficiency of XTP Methanocaldococcus jannaschii IMP + diphosphate
-
 ?
3.6.1.66 ITP + H2O catalytic efficiency (kcat/Km) is about 60% compared to the catalytic efficiency of XTP Methanocaldococcus jannaschii DSM 2661 IMP + diphosphate
-
 ?
3.6.1.66 additional information catalytic efficiency (kcat/Km) for GTP, dGTP, ATP, CTP or TTP is less than 1% compared to the catalytic efficiency of XTP Methanocaldococcus jannaschii ?
-
 ?
3.6.1.66 additional information catalytic efficiency (kcat/Km) for GTP, dGTP, ATP, CTP or TTP is less than 1% compared to the catalytic efficiency of XTP Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
3.6.1.66 XTP + H2O
-
 Methanocaldococcus jannaschii XMP + diphosphate
-
 ?
3.6.1.66 XTP + H2O
-
 Methanocaldococcus jannaschii DSM 2661 XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.66 homodimer
-
 Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.6.1.66 Mj0226 locus name Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.66 80
-
 assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.66 911.7
-
 ITP pH 7.6, 80°C Methanocaldococcus jannaschii
3.6.1.66 1009
-
 XTP pH 7.6, 80°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.66 7.6
-
 assay at Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.66 5998
-
 ITP pH 7.6, 80°C Methanocaldococcus jannaschii
3.6.1.66 10200
-
 XTP pH 7.6, 80°C Methanocaldococcus jannaschii