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Literature summary extracted from

  • Hagelueken, G.; Huang, H.; Clarke, B.R.; Lebl, T.; Whitfield, C.; Naismith, J.H.
    Structure of WbdD: a bifunctional kinase and methyltransferase that regulates the chain length of the O antigen in Escherichia coli O9a (2012), Mol. Microbiol., 86, 730-742.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.181
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.181 2.2 A resolution structure reveals a bacterial methyltransferase domain joined to a eukaryotic kinase domain. The kinase domain is again fused to an extended C-terminal coiled-coil domain reminiscent of eukaryotic Myotonic Dystrophy Protein Kinase family kinases. Structures of co-complexes with two known eukaryotic protein kinase inhibitors which are potent inhibitors in vitro, but do not show any in vivo activity Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.181 D350A 88.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 95.2% with substrate D-mannose Escherichia coli
2.7.1.181 D351A 0.9% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.1% with substrate D-mannose Escherichia coli
2.7.1.181 D351E 4.5% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.5% with substrate D-mannose Escherichia coli
2.7.1.181 DELTA398-418 6.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 4.7% with substrate D-mannose Escherichia coli
2.7.1.181 E274A 45.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 66.6% with substrate D-mannose Escherichia coli
2.7.1.181 H132A complete loss of activity Escherichia coli
2.7.1.181 H133A complete loss of activity Escherichia coli
2.7.1.181 N34D/Q35E/H197E complete loss of activity Escherichia coli
2.7.1.181 R203A complete loss of activity Escherichia coli
2.7.1.181 R270A 72.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 85.7% with substrate D-mannose Escherichia coli
2.7.1.181 W355F 72.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.9% with substrate D-mannose Escherichia coli
2.7.1.181 W355H 48.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 50.7% with substrate D-mannose Escherichia coli
2.7.1.181 Y16F complete loss of activity Escherichia coli
2.7.1.181 Y230F 0.1% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 2.5% with substrate D-mannose Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.181 Escherichia coli J7I4B7
-
-
2.7.1.181 Escherichia coli O9a J7I4B7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.181 ATP + 2-alpha-D-mannosyl-D-mannose
-
 Escherichia coli ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose
-
 ?
2.7.1.181 ATP + 2-alpha-D-mannosyl-D-mannose
-
 Escherichia coli O9a ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose
-
 ?
2.7.1.181 ATP + D-mannose
-
 Escherichia coli ADP + 3-O-phospho-D-mannose
-
 ?
2.7.1.181 ATP + D-mannose
-
 Escherichia coli O9a ADP + 3-O-phospho-D-mannose
-
 ?