EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.181 | - |
Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.181 | 2.2 A resolution structure reveals a bacterial methyltransferase domain joined to a eukaryotic kinase domain. The kinase domain is again fused to an extended C-terminal coiled-coil domain reminiscent of eukaryotic Myotonic Dystrophy Protein Kinase family kinases. Structures of co-complexes with two known eukaryotic protein kinase inhibitors which are potent inhibitors in vitro, but do not show any in vivo activity | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.181 | D350A | 88.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 95.2% with substrate D-mannose | Escherichia coli |
2.7.1.181 | D351A | 0.9% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.1% with substrate D-mannose | Escherichia coli |
2.7.1.181 | D351E | 4.5% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.5% with substrate D-mannose | Escherichia coli |
2.7.1.181 | DELTA398-418 | 6.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 4.7% with substrate D-mannose | Escherichia coli |
2.7.1.181 | E274A | 45.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 66.6% with substrate D-mannose | Escherichia coli |
2.7.1.181 | H132A | complete loss of activity | Escherichia coli |
2.7.1.181 | H133A | complete loss of activity | Escherichia coli |
2.7.1.181 | N34D/Q35E/H197E | complete loss of activity | Escherichia coli |
2.7.1.181 | R203A | complete loss of activity | Escherichia coli |
2.7.1.181 | R270A | 72.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 85.7% with substrate D-mannose | Escherichia coli |
2.7.1.181 | W355F | 72.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.9% with substrate D-mannose | Escherichia coli |
2.7.1.181 | W355H | 48.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 50.7% with substrate D-mannose | Escherichia coli |
2.7.1.181 | Y16F | complete loss of activity | Escherichia coli |
2.7.1.181 | Y230F | 0.1% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 2.5% with substrate D-mannose | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.181 | Escherichia coli | J7I4B7 | - |
- |
2.7.1.181 | Escherichia coli O9a | J7I4B7 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.181 | ATP + 2-alpha-D-mannosyl-D-mannose | - |
Escherichia coli | ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose | - |
? | |
2.7.1.181 | ATP + 2-alpha-D-mannosyl-D-mannose | - |
Escherichia coli O9a | ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose | - |
? | |
2.7.1.181 | ATP + D-mannose | - |
Escherichia coli | ADP + 3-O-phospho-D-mannose | - |
? | |
2.7.1.181 | ATP + D-mannose | - |
Escherichia coli O9a | ADP + 3-O-phospho-D-mannose | - |
? |