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Literature summary extracted from

  • Bandini, G.; Marino, K.; Guether, M.L.; Wernimont, A.K.; Kuettel, S.; Qiu, W.; Afzal, S.; Kelner, A.; Hui, R.; Ferguson, M.A.
    Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase (2012), Mol. Microbiol., 85, 513-534.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.3 gene PAGM, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma brucei
5.4.2.8 gene PMM, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.8 purified recombinant detagged enzyme, hanging drop vapour diffusion, from 2 M (NH4)2SO4, 0.2 M NaCl, 0.1 M sodium cacodylate, pH 6.0, 20°C, X-ray diffraction structure determination and analysis at 1.86 A resolution, molecular replacement using a modified form of the Leishmania mexicana enzyme, PDB ID 2i54 Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.3 additional information RNAi knockdown of gene TbPAGM, construction of a cell line expressing tetracycline inducible double-stranded RNA targeting TbPMM, induction of dsRNA targeting TbPMM results in a 68% knockdown of TbPMM mRNA after 48 h and a reduction of growth rate that leads to cell death after 72 h, phenotypes, overview Trypanosoma brucei
5.4.2.8 additional information RNAi knockdown of gene TbPMM, construction of a cell line expressing tetracycline inducible double-stranded RNA targeting TbPMM, induction of dsRNA targeting TbPMM results in a 68% knockdown of TbPMM mRNA after 48 h and a reduction of growth rate that leads to cell death after 72 h, phenotypes, overview Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.3 D-glucose 1-phosphate competitive inhibition Trypanosoma brucei
5.4.2.3 D-glucose 6-phosphate competitive inhibition Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.3 0.014
-
 N-acetyl-D-glucosamine 6-phosphate pH 7.3, 37°C Trypanosoma brucei
5.4.2.8 0.327
-
 alpha-D-mannose 1-phosphate pH 7.3, 37°C Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.2.3 cytosol
-
 Trypanosoma brucei 5829
-
5.4.2.3 glycosome
-
 Trypanosoma brucei 20015
-
5.4.2.8 glycosome blood stream parasite form Trypanosoma brucei 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.3 Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.3 additional information Trypanosoma brucei the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?
5.4.2.3 additional information Trypanosoma brucei 427 the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate Trypanosoma brucei
-
 N-acetyl-D-glucosamine 6-phosphate
-
 r
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate Trypanosoma brucei 427
-
 N-acetyl-D-glucosamine 6-phosphate
-
 r
5.4.2.8 alpha-D-mannose 1-phosphate Trypanosoma brucei
-
 D-mannose 6-phosphate
-
 ?
5.4.2.8 alpha-D-mannose 1-phosphate Trypanosoma brucei 427
-
 D-mannose 6-phosphate
-
 ?
5.4.2.8 additional information Trypanosoma brucei the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?
5.4.2.8 additional information Trypanosoma brucei 427 the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 no activity in Trypanosoma brucei
-
 the organism lost the PGM gene, activity is catalyzed by phosphomannomutase, EC 5.4.2.8 and phospho-N-acetylglucosamine mutase, EC 5.4.2.3
-
5.4.2.3 Trypanosoma brucei F4NCC3 gene PAGM
-
5.4.2.3 Trypanosoma brucei 427 F4NCC3 gene PAGM
-
5.4.2.8 Trypanosoma brucei F4NCC2 gene PMM
-
5.4.2.8 Trypanosoma brucei 427 F4NCC2 gene PMM
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.3 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Trypanosoma brucei
5.4.2.8 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag by TEV protease Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.3 additional information quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms Trypanosoma brucei
-
5.4.2.8 additional information quantitative proteomic analysis show that the TbPMM protein is present in both life cycle stages, procyclic and bloodstream forms Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.3 additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei ?
-
 ?
5.4.2.3 additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei 427 ?
-
 ?
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Trypanosoma brucei N-acetyl-D-glucosamine 6-phosphate
-
 r
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Trypanosoma brucei 427 N-acetyl-D-glucosamine 6-phosphate
-
 r
5.4.2.3 N-acetyl-D-glucosamine 6-phosphate
-
 Trypanosoma brucei N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
5.4.2.3 N-acetyl-D-glucosamine 6-phosphate
-
 Trypanosoma brucei 427 N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
5.4.2.8 alpha-D-mannose 1-phosphate
-
 Trypanosoma brucei D-mannose 6-phosphate
-
 ?
5.4.2.8 alpha-D-mannose 1-phosphate
-
 Trypanosoma brucei 427 D-mannose 6-phosphate
-
 ?
5.4.2.8 additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei ?
-
 ?
5.4.2.8 additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei 427 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.3 PAGM
-
 Trypanosoma brucei
5.4.2.8 PMM
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.3 37
-
 assay at Trypanosoma brucei
5.4.2.8 37
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.3 7.2 9.1 broad optimum Trypanosoma brucei
5.4.2.8 6.5
-
-
 Trypanosoma brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.2.8 4 8 activity range, inactive above and below Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.4.2.3 0.0063
-
 versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C Trypanosoma brucei D-glucose 1-phosphate
5.4.2.3 0.0083
-
 versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C Trypanosoma brucei D-glucose 6-phosphate

General Information

EC Number General Information Comment Organism
5.4.2.3 evolution the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes Trypanosoma brucei
5.4.2.3 physiological function the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei
5.4.2.8 evolution the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes Trypanosoma brucei
5.4.2.8 additional information the cap domain contributes the residues involved in substrate recognition, E119, R121, M124, N126, R132, R139, S177, so that the active site is located in the groove at the interface of the core and cap domains Trypanosoma brucei
5.4.2.8 physiological function the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphoacetylglucosamine mutase, EC 5.4.2.3, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.2.3 16
-
 N-acetyl-D-glucosamine 6-phosphate pH 7.3, 37°C Trypanosoma brucei
5.4.2.8 37
-
 alpha-D-mannose 1-phosphate pH 7.3, 37°C Trypanosoma brucei