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Literature summary extracted from
- Control of hydroxyproline catabolism in Sinorhizobium meliloti (2012), Mol. Microbiol., 85, 1133-1147.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.8 | gene hypRE, encoded in the Hyp catabolism locus, DNA and amino acid sequence determination and analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21/DE3/pET28 from the T7 promoter | Sinorhizobium meliloti |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.8 | additional information | - |
additional information | Michaelis-Menten kinetics | Sinorhizobium meliloti | |
| 5.1.1.8 | 8 | - |
cis-4-hydroxy-D-proline | pH not specified in the publication, 30°C, recombinant enzyme | Sinorhizobium meliloti |  |
| 5.1.1.8 | 10 | - |
trans-4-hydroxy-L-proline | pH not specified in the publication, 30°C, recombinant enzyme | Sinorhizobium meliloti | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.8 | trans-4-hydroxy-L-proline | Sinorhizobium meliloti | - |
cis-4-hydroxy-D-proline | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.8 | Sinorhizobium meliloti | - |
gene hypRE or smb20268 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.8 | trans-4-hydroxy-L-proline | - |
Sinorhizobium meliloti | cis-4-hydroxy-D-proline | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.1.8 | 4-hydroxyproline 2-epimerase | - |
Sinorhizobium meliloti |
| 5.1.1.8 | HyPRE | - |
Sinorhizobium meliloti |
| 5.1.1.8 | Smb20268 | - |
Sinorhizobium meliloti |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.8 | 25 | 30 | assay at | Sinorhizobium meliloti |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 5.1.1.8 | Sinorhizobium meliloti | the enzyme is strongly induced by cis-4-hydroxy-D-proline, moderately by trans-4-hydroxy-L-proline | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.1.8 | malfunction | a hypRE mutant grows with cis-4-hydroxy-D-proline but not with trans-4-hydroxy-L-proline, and it exhibits wild-type growth on succinate and proline, mutant complementation with the native smb20268 gene | Sinorhizobium meliloti |
| 5.1.1.8 | metabolism | the enzyme performs the first step of hydroxyproline catabolism, regulation, hyp cluster promoters are regulated by HypR, overview. cis-4-Hydroxy-D-proline is the effector that interacts with HypR and the results suggest a role of positive feedback in the regulation of hyp catabolism, as cis-4-hydroxy-D-proline (or a downstream catabolite) is a stronger inducer than the initial substrate trans-4-hydroxy-L-proline | Sinorhizobium meliloti |
| 5.1.1.8 | additional information | the reaction mechanism requires two active site cysteines | Sinorhizobium meliloti |
| 5.1.1.8 | physiological function | the enzyme is absolutely required for growth on trans-4-hydroxy-L-proline | Sinorhizobium meliloti |
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