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Literature summary extracted from
- A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting beta-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c (2012), Microb. Cell Fact., 11, 113.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | expressed in Pichia pastoris and in Escherichia coli strain BL21(DE3)pLysS | Arthrobacter sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | Co2+ | 82% residual activity at 10 mM | Arthrobacter sp. |  |
| 5.3.1.4 | additional information | no activity loss is observed after treatment with EDTA for 12 h | Arthrobacter sp. | |
| 5.3.1.4 | Ni2+ | 70% residual activity at 10 mM | Arthrobacter sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 119 | - |
D-galactose | at pH 7.0 and 50°C | Arthrobacter sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | additional information | the enzyme is not dependent on divalent metal ions, since it is only marginally activated by Mg2+, Mn2+ or Ca2+ | Arthrobacter sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 55000 | - |
6 * 55000, SDS-PAGE | Arthrobacter sp. |
| 5.3.1.4 | 55183 | - |
6 * 55183, calculated from amino acid sequence | Arthrobacter sp. |
| 5.3.1.4 | 335000 | - |
gel filtration | Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | L-arabinose | Arthrobacter sp. | - |
L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | Arthrobacter sp. 22c | - |
L-ribulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.4 | Arthrobacter sp. | H6UGW9 | - |
- |
| 5.3.1.4 | Arthrobacter sp. 22c | H6UGW9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | Fractogel EMD DEAE chromatography and Fractogel EMD TMAE chromatography | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 0.026 | - |
cell extract, at pH 7.5 and 52°C | Arthrobacter sp. |
| 5.3.1.4 | 0.232 | - |
after 8.9fold purification, at pH 7.5 and 52°C | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | D-galactose | - |
Arthrobacter sp. | D-tagatose | 30% yield after 36 h at 50°C | ? | |
| 5.3.1.4 | D-galactose | - |
Arthrobacter sp. 22c | D-tagatose | 30% yield after 36 h at 50°C | ? | |
| 5.3.1.4 | L-arabinose | - |
Arthrobacter sp. | L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Arthrobacter sp. 22c | L-ribulose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | homohexamer | 6 * 55000, SDS-PAGE | Arthrobacter sp. |
| 5.3.1.4 | homohexamer | 6 * 55183, calculated from amino acid sequence | Arthrobacter sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 52 | - |
- |
Arthrobacter sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 30 | - |
the enzyme exhibits over 60% of maximum activity at 30°C | Arthrobacter sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 50 | 70 | over 90% of the initial activity is retained after 72 h incubation at 50°C. At 60 and 65°C, over 50% of initial activity is lost in incubations of 60 and 30 min, respectively. The enzyme is completely inactivated by 15 min at 70°C | Arthrobacter sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 8 | - |
- |
Arthrobacter sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 5 | 9 | the enzyme maintains over 90% of maximum activity in a pH range of 5.0 to 9.0 | Arthrobacter sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 7 | - |
over 90% of the initial activity is retained after 72 h incubation at pH 7.0 | Arthrobacter sp. |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.3.1.4 | Arthrobacter sp. | calculated from amino acid sequence | - |
5.1 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 0.0023 | - |
D-galactose | at pH 7.0 and 50°C | Arthrobacter sp. | |
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